SCOP Antibody - #DF8606

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Product: SCOP Antibody
Catalog: DF8606
Description: Rabbit polyclonal antibody to SCOP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 184 kDa; 185kD(Calculated).
Uniprot: O60346
RRID: AB_2841810

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Horse(89%), Rabbit(89%)
Clonality:
Polyclonal
Specificity:
SCOP Antibody detects endogenous levels of total SCOP.
RRID:
AB_2841810
Cite Format: Affinity Biosciences Cat# DF8606, RRID:AB_2841810.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hSCOP; KIAA0606; PH domain and leucine rich repeat protein phosphatase; PH domain leucine rich repeat containing protein phosphatase; PH domain leucine rich repeat protein phosphatase; PH domain leucine-rich repeat-containing protein phosphatase 1; PH domain-containing family E member 1; PHLP1_HUMAN; PHLPP; Phlpp1; Pleckstrin homology domain containing family E (with leucine rich repeats) member 1; Pleckstrin homology domain containing family E protein 1; Pleckstrin homology domain-containing family E member 1; PLEKHE 1 protein; PLEKHE1; SCN circadian oscillatory protein; SCOP; Suprachiasmatic nucleus circadian oscillatory protein; Suprachiasmatic nucleus circadian protein;

Immunogens

Immunogen:
Uniprot:

O60346(PHLP1_HUMAN) >>Visit HPA database.

Gene(ID):
PHLPP1(23239)
Expression:
O60346 PHLP1_HUMAN:

In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.

Sequence:
MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Rabbit
89
Bovine
78
Dog
78
Chicken
67
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60346 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S118 Phosphorylation
S131 Phosphorylation
S144 Phosphorylation
T169 Phosphorylation
T184 Phosphorylation
K240 Ubiquitination
K246 Ubiquitination
S279 Phosphorylation
S313 Phosphorylation
S317 Phosphorylation
S321 Phosphorylation
S323 Phosphorylation
S324 Phosphorylation
S336 Phosphorylation
S412 Phosphorylation
S425 Phosphorylation
S450 Phosphorylation
T451 Phosphorylation
K1071 Ubiquitination
K1146 Ubiquitination
K1323 Ubiquitination
S1359 Phosphorylation P49841 (GSK3B) , P48729 (CSNK1A1)
T1363 Phosphorylation P49841 (GSK3B) , P48729 (CSNK1A1)
S1379 Phosphorylation P48729 (CSNK1A1) , P49841 (GSK3B)
S1381 Phosphorylation P48729 (CSNK1A1) , P49841 (GSK3B)
K1400 Ubiquitination
S1526 Phosphorylation
T1617 Phosphorylation
Y1712 Phosphorylation
Y1713 Phosphorylation

Research Backgrounds

Function:

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (By similarity). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (By similarity). Involved in development and function of regulatory T-cells (By similarity).

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein. Nucleus.
Note: In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.

Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.

Subunit Structure:

Interacts with the nucleotide free form of K-Ras (KRAS) via its LRR repeats (By similarity). Interacts with AKT2, AKT3, PRKCB isoform beta-II, STK4, RPS6KB1, RAF1. Isoform 1 (predominantly) and isoform 2 interact with BRAP. Interacts with FKBP5; FKBP5 acts as a scaffold for PHLPP1 and Akt. Interacts with SCRIB; SCRIB acts as a scaffold for PHLPP1 and Akt. Interacts with SLC9A3R1; SLC9A3R1 scaffolds a heterotrimeric complex with PTEN at the plasma membrane. Interacts with WDR48 and USP12.

Family&Domains:

The PH domain is required for interaction with PRKCB and its dephosphorylation.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

References

1). RNA-binding Protein Trinucleotide repeat-containing 6A Regulates the Formation of Circular RNA 0006916, with Important Functions in Lung Cancer Cells. CARCINOGENESIS, 2018 (PubMed: 29726904) [IF=4.7]

Application: WB    Species: human    Sample: 16HBE-T, A549 and H460 cellls

Fig. 4. |Circ0006916 served as a sponge for has-miR-522-3p.(F) PHLPP1 expression levels in cells detected by qPCR (**P < 0.01, compared with 16HBE). (G) PHLPP1 mRNA levels after transfection with siCirc and oeCirc. (*P < 0.05, **P < 0.01, compared with control siRNA and empty vector). (H, I) Protein expression levels. (*P < 0.05, **P<0.01).

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