Sarcoglycan beta Antibody - #DF8605

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Product: Sarcoglycan beta Antibody
Catalog: DF8605
Description: Rabbit polyclonal antibody to Sarcoglycan beta
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 kDa; 35kD(Calculated).
Uniprot: Q16585
RRID: AB_2841809

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Sarcoglycan beta Antibody detects endogenous levels of total Sarcoglycan beta.
RRID:
AB_2841809
Cite Format: Affinity Biosciences Cat# DF8605, RRID:AB_2841809.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

43 kDa dystrophin associated glycoprotein; 43 kDa dystrophin-associated glycoprotein; 43DAG; A3b; Beta SG; Beta-sarcoglycan; Beta-SG; dystrophin-associated glycoprotein, 43-kD; LGMD2E; Limb girdle muscular dystrophy 2E (non linked families); Sarcoglycan beta; sarcoglycan, beta (43kDa dystrophin-associated glycoprotein); SGC; SGCB; SGCB_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q16585(SGCB_HUMAN) >>Visit HPA database.

Gene(ID):
SGCB(6443)
Expression:
Q16585 SGCB_HUMAN:

Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.

Sequence:
MAAAAAAAAEQQSSNGPVKKSMREKAVERRSVNKEHNSNFKAGYIPIDEDRLHKTGLRGRKGNLAICVIILLFILAVINLIITLVIWAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGNEGVFIMGKTIEFHMGGNMELKAENSIILNGSVMVSTTRLPSSSSGDQLGSGDWVRYKLCMCADGTLFKVQVTSQNMGCQISDNPCGNTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16585 As Substrate

Site PTM Type Enzyme
K34 Ubiquitination
K41 Ubiquitination
Y44 Phosphorylation
K111 Ubiquitination
K124 Ubiquitination
K152 Ubiquitination
N158 N-Glycosylation
K196 Ubiquitination
K202 Ubiquitination
N211 N-Glycosylation
K219 Ubiquitination
T266 Phosphorylation
K286 Ubiquitination

Research Backgrounds

Function:

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

PTMs:

Disulfide bonds are present.

Subcellular Location:

Cell membrane>Sarcolemma>Single-pass type II membrane protein. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.

Subunit Structure:

Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans (By similarity).

Family&Domains:

Belongs to the sarcoglycan beta/delta/gamma/zeta family.

Research Fields

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

Restrictive clause

 

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For Research Use Only.
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