RNase III Drosha Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse testis, using RNase III Drosha Antibody at 1/1000 dilution.

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using RNase III Drosha Antibody at 1/1000 dilution.

Product:	RNase III Drosha Antibody
Catalog:	DF8601
Description:	Rabbit polyclonal antibody to RNase III Drosha
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.:	159 kDa; 159kD(Calculated).
Uniprot:	Q9NRR4
RRID:	AB_2841805

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

RNase III Drosha Antibody detects endogenous levels of total RNase III Drosha.

RRID:

AB_2841805
Cite Format: Affinity Biosciences Cat# DF8601, RRID:AB_2841805.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DROSHA; Drosha double stranded RNA specific endoribonuclease; Drosha ribonuclease type III; Etohi2; HSA242976; Nuclear RNase III Drosha; p241; Protein Drosha; Putative protein p241 which interacts with transcription factor Sp1; Putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; Ribonuclease III nuclear; Ribonuclease type III nuclear; RibonucleaseIII; RN 3; RN3; RNase 3; RNase III; RNase3; RNASE3L; RNaseIII; RNASEN; RNC_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9NRR4(RNC_HUMAN) >>Visit HPA database.

Gene(ID):

DROSHA(29102)

Expression:

Q9NRR4 RNC_HUMAN:

Ubiquitous.

Sequence:

Q9NRR4 RNC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQGTFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRSYERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEIVNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Zebrafish

100

Chicken

100

Rabbit

100

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRR4 As Substrate

Q9NRR4

Site	PTM Type	Enzyme	Source
S221	Phosphorylation	Q16539 (MAPK14)	Uniprot
Y228	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
S255	Phosphorylation	Q16539 (MAPK14)	Uniprot
S267	Phosphorylation		Uniprot
Y269	Phosphorylation		Uniprot
R271	Methylation		Uniprot
T274	Phosphorylation	Q16539 (MAPK14)	Uniprot
S276	Phosphorylation		Uniprot
S300	Phosphorylation	Q16539 (MAPK14) , P49841 (GSK3B)	Uniprot
S302	Phosphorylation	P49841 (GSK3B)	Uniprot
S317	Phosphorylation		Uniprot
S355	Phosphorylation	Q16539 (MAPK14)	Uniprot
S357	Phosphorylation		Uniprot
S373	Phosphorylation		Uniprot
K382	Ubiquitination		Uniprot
K388	Sumoylation		Uniprot
K409	Sumoylation		Uniprot
K409	Ubiquitination		Uniprot
K447	Ubiquitination		Uniprot
S491	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
Y705	Phosphorylation		Uniprot
K738	Ubiquitination		Uniprot
T747	Phosphorylation		Uniprot
K748	Ubiquitination		Uniprot
K792	Ubiquitination		Uniprot
K795	Ubiquitination		Uniprot
S796	Phosphorylation		Uniprot
S807	Phosphorylation		Uniprot
K809	Ubiquitination		Uniprot
K827	Ubiquitination		Uniprot
K831	Ubiquitination		Uniprot
K1207	Ubiquitination		Uniprot
K1250	Ubiquitination		Uniprot
K1262	Ubiquitination		Uniprot
K1277	Ubiquitination		Uniprot
K1285	Ubiquitination		Uniprot
S1293	Phosphorylation		Uniprot
K1313	Ubiquitination		Uniprot
K1331	Ubiquitination		Uniprot
K1341	Ubiquitination		Uniprot
K1353	Ubiquitination		Uniprot
K1373	Ubiquitination		Uniprot

Research Backgrounds

Q9NRR4_RNC_HUMAN

Function:

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with SP1 and SNIP1. Interacts with SRRT/ARS2 (By similarity). Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (By similarity).

Family&Domains:

The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.

Belongs to the ribonuclease III family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022 (PubMed: 36359855) [IF=6.0]

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RNase III Drosha Antibody - #DF8601