RFC3 Antibody - #DF8600

(3)  
Product: RFC3 Antibody
Catalog: DF8600
Description: Rabbit polyclonal antibody to RFC3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 kDa; 41kD(Calculated).
Uniprot: P40938
RRID: AB_2841804

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(82%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RFC3 Antibody detects endogenous levels of total RFC3.
RRID:
AB_2841804
Cite Format: Affinity Biosciences Cat# DF8600, RRID:AB_2841804.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A1 38 kDa subunit; Activator 1 38 kDa subunit; Activator 1 subunit 3; HGNC:9971; MGC5276; Replication factor C (activator 1) 3; Replication factor C (activator 1) 3, 38kDa; Replication factor C 3; Replication factor C 38 kDa subunit; Replication factor C subunit 3; RF-C 38 kDa subunit; RFC 38 kD subunit; RFC 38 kDa subunit; RFC3; RFC3_HUMAN; RFC38;

Immunogens

Immunogen:
Uniprot:

P40938(RFC3_HUMAN) >>Visit HPA database.

Gene(ID):
RFC3(5983)
Sequence:
MSLWVDKYRPCSLGRLDYHKEQAAQLRNLVQCGDFPHLLVYGPSGAGKKTRIMCILRELYGVGVEKLRIEHQTITTPSKKKIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQSQQLETNSQRDFKVVLLTEVDKLTKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSRCLAVRVPAPSIEDICHVLSTVCKKEGLNLPSQLAHRLAEKSCRNLRKALLMCEACRVQQYPFTADQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDGLEGMMF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Zebrafish
82
Chicken
75
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40938 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K7 Methylation
K7 Ubiquitination
S12 Phosphorylation
K20 Acetylation
K20 Ubiquitination
K48 Ubiquitination
Y60 Phosphorylation
K66 Ubiquitination
T75 Phosphorylation
T76 Phosphorylation
K79 Ubiquitination
Y91 Phosphorylation
K113 Ubiquitination
T123 Phosphorylation
S125 Phosphorylation
K130 Ubiquitination
K139 Ubiquitination
K142 Ubiquitination
K154 Ubiquitination
K170 Ubiquitination
K201 Ubiquitination
K202 Ubiquitination
S209 Phosphorylation
K218 Ubiquitination
K225 Ubiquitination
T283 Phosphorylation
Y343 Phosphorylation

Research Backgrounds

Function:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

Family&Domains:

Belongs to the activator 1 small subunits family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

Restrictive clause

 

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For Research Use Only.
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