Pragmin Antibody - #DF8595

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Product: Pragmin Antibody
Catalog: DF8595
Description: Rabbit polyclonal antibody to Pragmin
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 149 kDa; 150kD(Calculated).
Uniprot: Q86YV5
RRID: AB_2841799

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(83%)
Clonality:
Polyclonal
Specificity:
Pragmin Antibody detects endogenous levels of total Pragmin.
RRID:
AB_2841799
Cite Format: Affinity Biosciences Cat# DF8595, RRID:AB_2841799.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D8Ertd82e; DKFZp761P0423; DNA segment, Chr 8, ERATO Doi 82; EC 2.7.10.2; Homolog of rat pragma of Rnd2; LOC157285; PRAGMIN; SG223_HUMAN; Sgk223; Sugen kinase 223; Tyrosine protein kinase SgK223; Tyrosine-protein kinase SgK223;

Immunogens

Immunogen:
Uniprot:

Q86YV5(PRAG1_HUMAN) >>Visit HPA database.

Gene(ID):
PRAG1(157285)
Sequence:
MHQTLCLNPESLKMSACSDFVEHIWKPGSCKNCFCLRSDHQLVAGPPQPRAGSLPPPPRLPPRPENCRLEDEGVNSSPYSKPTIAVKPTMMSSEASDVWTEANLSAEVSQVIWRRAPGKLPLPKQEDAPVVYLGSFRGVQKPAGPSTSPDGNSRCPPAYTMVGLHNLEPRGERNIAFHPVSFPEEKAVHKEKPSFPYQDRPSTQESFRQKLAAFAGTTSGCHQGPGPLRESLPSEDDSDQRCSPSGDSEGGEYCSILDCCPGSPVAKAASQTAGSRGRHGGRDCSPTCWEQGKCSGPAEQEKRGPSFPKECCSQGPTAHPSCLGPKKLSLTSEAAISSDGLSCGSGSGSGSGASSPFVPHLESDYCSLMKEPAPEKQQDPGCPGVTPSRCLGLTGEPQPPAHPREATQPEPIYAESTKRKKAAPVPSKSQAKIEHAAAAQGQGQVCTGNAWAQKAASGWGRDSPDPTPQVSATITVMAAHPEEDHRTIYLSSPDSAVGVQWPRGPVSQNSEVGEEETSAGQGLSSRESHAHSASESKPKERPAIPPKLSKSSPVGSPVSPSAGGPPVSPLADLSDGSSGGSSIGPQPPSQGPADPAPSCRTNGVAISDPSRCPQPAASSASEQRRPRFQAGTWSRQCRIEEEEEVEQELLSHSWGRETKNGPTDHSNSTTWHRLHPTDGSSGQNSKVGTGMSKSASFAFEFPKDRSGIETFSPPPPPPKSRHLLKMNKSSSDLEKVSQGSAESLSPSFRGVHVSFTTGSTDSLASDSRTCSDGGPSSELAHSPTNSGKKLFAPVPFPSGSTEDVSPSGPQQPPPLPQKKIVSRAASSPDGFFWTQGSPKPGTASPKLNLSHSETNVHDESHFSYSLSPGNRHHPVFSSSDPLEKAFKGSGHWLPAAGLAGNRGGCGSPGLQCKGAPSASSSQLSVSSQASTGSTQLQLHGLLSNISSKEGTYAKLGGLYTQSLARLVAKCEDLFMGGQKKELHFNENNWSLFKLTCNKPCCDSGDAIYYCATCSEDPGSTYAVKICKAPEPKTVSYCSPSVPVHFNIQQDCGHFVASVPSSMLSSPDAPKDPVPALPTHPPAQEQDCVVVITREVPHQTASDFVRDSAASHQAEPEAYERRVCFLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHCTLQAGPGPAPAPAPAPAPAAAAPPCSSAAPPAGGTLSPAAGPASPEGPREKQLPRLIISNFLKAKQKPGGTPNLQQKKSQARLAPEIVSASQYRKFDEFQTGILIYELLHQPNPFEVRAQLRERDYRQEDLPPLPALSLYSPGLQQLAHLLLEADPIKRIRIGEAKRVLQCLLWGPRRELVQQPGTSEEALCGTLHNWIDMKRALMMMKFAEKAVDRRRGVELEDWLCCQYLASAEPGALLQSLKLLQLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Pig
78
Dog
67
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86YV5 As Substrate

Site PTM Type Enzyme
S53 Phosphorylation
Y79 Phosphorylation
Y132 Phosphorylation
S135 Phosphorylation
S146 Phosphorylation
T147 Phosphorylation
S148 Phosphorylation
Y159 Phosphorylation
T160 Phosphorylation
Y197 Phosphorylation
T203 Phosphorylation
S234 Phosphorylation
Y253 Phosphorylation
S263 Phosphorylation
S285 Phosphorylation
S306 Phosphorylation
S313 Phosphorylation
T317 Phosphorylation
S321 Phosphorylation
S363 Phosphorylation
Y365 Phosphorylation
T386 Phosphorylation
Y413 Phosphorylation
S416 Phosphorylation
S463 Phosphorylation
Y489 Phosphorylation
S492 Phosphorylation
S495 Phosphorylation
S507 Phosphorylation
S510 Phosphorylation
T517 Phosphorylation
S610 Phosphorylation
T632 Phosphorylation
S651 Phosphorylation
S653 Phosphorylation
S668 Phosphorylation
K686 Acetylation
K693 Acetylation
S694 Phosphorylation
S696 Phosphorylation
T710 Phosphorylation
S712 Phosphorylation
S729 Phosphorylation
S730 Phosphorylation
S737 Phosphorylation
S740 Phosphorylation
S743 Phosphorylation
S745 Phosphorylation
S747 Phosphorylation
S759 Phosphorylation
S771 Phosphorylation
S776 Phosphorylation
S782 Phosphorylation
T784 Phosphorylation
S786 Phosphorylation
S798 Phosphorylation
S805 Phosphorylation
S807 Phosphorylation
S826 Phosphorylation
S827 Phosphorylation
S837 Phosphorylation
T842 Phosphorylation
S844 Phosphorylation
S852 Phosphorylation
T854 Phosphorylation
S863 Phosphorylation
Y864 Phosphorylation
S865 Phosphorylation
S867 Phosphorylation
S878 Phosphorylation
S879 Phosphorylation
S889 Phosphorylation
S907 Phosphorylation
T1227 Phosphorylation

Research Backgrounds

Function:

Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (By similarity).

PTMs:

Phosphorylated by CSK on Tyr-253, Tyr-365, and Tyr-413; Tyr-413 is a primary site of phosphorylation.

Subcellular Location:

Cytoplasm. Cell junction>Focal adhesion. Nucleus.
Note: Colocalized with NOTCH1 in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Dimerization leads to the catalytic activation of CSK (By similarity). Interacts (via C-terminus) with RND2 (By similarity). Interacts with CSK (via SH2 domain) in a Tyr-413 phosphorylation-dependent manner; this interaction potentiates kinase activity of CSK (By similarity). Interacts with PEAK1. Interacts with NOTCH1 intracellular domain (N1ICD) (By similarity). Forms a complex with N1ICD and MAML1, in a MAML1-dependent manner (By similarity).

Family&Domains:

The dimerization region encompasses helices both from the N- and C-terminal of the protein kinase domain.

Belongs to the protein kinase superfamily.

Restrictive clause

 

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