PGE synthase Antibody - #DF8592

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Product: PGE synthase Antibody
Catalog: DF8592
Description: Rabbit polyclonal antibody to PGE synthase
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 17 kDa; 17kD(Calculated).
Uniprot: O14684
RRID: AB_2841796

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(91%), Sheep(92%), Rabbit(100%), Dog(83%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
PGE synthase Antibody detects endogenous levels of total PGE synthase.
RRID:
AB_2841796
Cite Format: Affinity Biosciences Cat# DF8592, RRID:AB_2841796.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Glutathione S transferase 1 like 1; MGC10317; MGST IV; MGST1 L1; MGST1 like 1; MGST1-L1; MGST1L1; MGSTIV; Microsomal glutathione S transferase 1 like 1; Microsomal glutathione S-transferase 1-like 1; Microsomal prostaglandin E synthase 1; Microsomal prostaglandin E synthase 1; mPGES 1; MPGES; mPGES-1; p53 induced apoptosis protein 12; p53 induced gene 12; p53 induced gene 12 protein; p53-induced gene 12 protein; PGES; PIG 12; PIG12; PP 102; PP 1294; PP102; PP1294; Prostaglandin E synthase; PTGES; PTGES_HUMAN; TP53I12; Tumor protein p53 inducible protein 12;

Immunogens

Immunogen:
Uniprot:

O14684(PTGES_HUMAN) >>Visit HPA database.

Gene(ID):
PTGES(9536)
Sequence:
MPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVTYTLAQLPCASMALQILWEAARHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
92
Sheep
92
Horse
91
Chicken
91
Dog
83
Zebrafish
67
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the MAPEG family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Kiwi Root Extract Inhibits the Development of Endometriosis in Mice by Downregulating Inflammatory Factors. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 33574880)

Application: WB    Species: Mice    Sample: endometriotic tissue

Figure 6 The expression of COX-1, COX-2, and EP2 mRNA and the expression of COX-1, COX-2, mPGEs, EP2, and PGE2 protein in endometriotic tissue of different groups. (a) The relative expression level of COX-1, COX-2, PGE2, and EP2 protein was detected by immunohistochemical staining (×200); the scale bar represents 50 microns. (b) The relative expression level of COX-1, COX-2, and mPGEs protein was detected by western blot. Data are expressed as mean ± standard deviation from triplicate experiments. (c) The relative expression level of COX-1, COX-2, and EP2 mRNA was detected by RT-PCR (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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