OPA1 Antibody - #DF8587

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Product: OPA1 Antibody
Catalog: DF8587
Description: Rabbit polyclonal antibody to OPA1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 111kDa, 80-95kDa; 112kD(Calculated).
Uniprot: O60313
RRID: AB_2841791

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
OPA1 Antibody detects endogenous levels of total OPA1.
RRID:
AB_2841791
Cite Format: Affinity Biosciences Cat# DF8587, RRID:AB_2841791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dynamin like 120 kDa protein; Dynamin like 120 kDa protein, mitochondrial; Dynamin-like 120 kDa protein, form S1; FLJ12460; Juvenile kjer type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; opa1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog;

Immunogens

Immunogen:
Uniprot:

O60313(OPA1_HUMAN) >>Visit HPA database.

Gene(ID):
OPA1(4976)
Expression:
O60313 OPA1_HUMAN:

Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

Sequence:
MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLKLQRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLLKLRYLILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDFEKIRKALPSSEDLVKLAPDFDKIVESLSLLKDFFTSGSPEETAFRATDRGSESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Horse
92
Xenopus
83
Zebrafish
83
Chicken
83
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60313 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
S22 Phosphorylation
S107 Phosphorylation
T114 Phosphorylation
K117 Ubiquitination
K123 Ubiquitination
Y132 Phosphorylation
K154 Ubiquitination
K164 Ubiquitination
K214 Ubiquitination
K228 Acetylation
K228 Ubiquitination
Y268 Phosphorylation
S298 Phosphorylation
K301 Ubiquitination
T302 Phosphorylation
S344 Phosphorylation
S345 Phosphorylation
T351 Phosphorylation
K352 Ubiquitination
S383 Phosphorylation
K387 Ubiquitination
T405 Phosphorylation
S440 Phosphorylation
K474 Ubiquitination
S478 Phosphorylation
K489 Ubiquitination
K527 Ubiquitination
K530 Ubiquitination
K535 Ubiquitination
K568 Ubiquitination
Y582 Phosphorylation
K598 Ubiquitination
S607 Phosphorylation
T612 Phosphorylation
K663 Ubiquitination
S682 Phosphorylation
K699 Ubiquitination
S708 Phosphorylation
K713 Ubiquitination
R733 Methylation
K772 Acetylation
Y808 Phosphorylation
T815 Phosphorylation
T816 Phosphorylation
R824 Methylation
S831 Phosphorylation
K834 Acetylation
K834 Ubiquitination
K911 Ubiquitination
K923 Ubiquitination
K926 Acetylation
T929 Phosphorylation
K931 Acetylation
K940 Ubiquitination
K948 Ubiquitination

Research Backgrounds

Function:

Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes. Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance.

Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.

Isoforms that contain the alternative exon 4b (present in isoform 4 and isoform 5) are required for mitochondrial genome maintenance, possibly by anchoring the mitochondrial nucleoids to the inner mitochondrial membrane.

PTMs:

PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.

Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ). This motif is present in isoform 2, isoform 3, isoform 4 and isoform 7, but is absent in the displayed isoform 1 and in isoform 5.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein. Mitochondrion intermembrane space. Mitochondrion membrane.
Note: Detected at contact sites between endoplamic reticulum and mitochondrion membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.

Subunit Structure:

Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with RCC1L; this interaction is direct. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Binds PARL (By similarity). Interacts with PRELID1.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

References

1). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024 (PubMed: 39613048) [IF=7.1]
2). Drp1-dependent mitochondrial fission contributes to Cr (VI)-induced mitophagy and hepatotoxicity. Ecotoxicology and Environmental Safety, 2020 (PubMed: 32888618) [IF=6.2]

Application: WB    Species: human    Sample: hepatocytes

Fig. 1.| Cr(VI) induced mitochondrial fission in the hepatocytes. (C) The protein expressions of the five molecules control the fusion/fission process, OPA1, MFN1, MFN2, FIS1, and Drp1 were assayed using western blotting. Mitochondrial protein was also extracted to determine the protein level of Drp1. VDAC1 (32 kDa) was the internal standard for the mitochondrial protein.
3). FGFR2 Mutation p.Cys342Arg Enhances Mitochondrial Metabolism-Mediated Osteogenesis via FGF/FGFR-AMPK-Erk1/2 Axis in Crouzon Syndrome. Cells, 2022 (PubMed: 36231091) [IF=6.0]

Application: WB    Species: Human    Sample:

Figure 7 Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
4). Valproic acid regulates MIEF1 through MST2-HIPPO to suppress breast cancer growth. Life sciences, 2022 (PubMed: 36126724) [IF=5.2]
5). Ginsenoside CK improves skeletal muscle insulin resistance by activating DRP1/PINK1-mediated mitophagy. Food & Function, 2023 (PubMed: 36562271) [IF=5.1]
6). Rostellularia procumbens (L) Nees. extract attenuates adriamycin-induced nephropathy by maintaining mitochondrial dynamics balance via SIRT1/PGC-1α signaling pathway activation. Journal of ethnopharmacology, 2025 (PubMed: 39733803) [IF=4.8]
7). Low-dose atorvastatin protects skeletal muscle mitochondria in high-fat diet-fed mice with mitochondrial autophagy inhibition and fusion enhancement. European journal of pharmacology, 2023 (PubMed: 37806539) [IF=4.2]

Application: WB    Species: Mouse    Sample:

Fig. 4. Ator treatment regulates expression of the proteins related to mitochondrial quality-control system in the skeletal muscles of mice. (A) Representative immunoblots for PINK1, LC3, PGC-1α, TFAM, and GAPDH protein in indicated skeletal muscles; (B–E) Quantification of PINK1, LC3, PGC-1α, and TFAM by densitometric analysis (n = 12); (F) Representative immunoblots for MFN1, MFN2, OPA1, DRP1, and GAPDH protein in indicated skeletal muscles; (G–J) Quantification of MFN1, MFN2, OPA1, and DRP1 by densitometric analysis (n = 12); (K) Correlation analysis between relative proteins expression (36 samples/variable). All data are shown as Mean ± SEM.
8). Glucose fluctuation accelerates cardiac injury of diabetic mice via sodium-dependent glucose cotransporter 1 (SGLT1). ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 34153296) [IF=3.8]

Application: WB    Species: Mice    Sample: heart

Fig. 7. SGLT1 knockdown promoted mitochondrial fusion and suppressed mitochondrial fission in heart of FDM mice. (A) The expression levels of mitochondrial fusion-related proteins (Mfn1, Mfn2, and Opa1) and fission-related proteins (Drp1 and Fis1) were detected by Western blot analysis. (B) Quantitative analysis of proteins in A was presented. Data were presented as mean ± SD (n = 6), and were compared with one-way ANOVA followed by post hoc Tukey’s test.
9). Knockdown of SGLT1 prevents the apoptosis of cardiomyocytes induced by glucose fluctuation via relieving oxidative stress and mitochondrial dysfunction. Biochemistry and Cell Biology, 2021 (PubMed: 33259229) [IF=2.4]
10). GSDMD induces hepatocyte pyroptosis to trigger alcoholic hepatitis through modulating mitochondrial dysfunction. Cell division, 2024 (PubMed: 38532477) [IF=2.3]
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