NET-5 Antibody - #DF8578

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Product: NET-5 Antibody
Catalog: DF8578
Description: Rabbit polyclonal antibody to NET-5
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 26 kDa; 27kD(Calculated).
Uniprot: O75954
RRID: AB_2841782

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(86%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NET-5 Antibody detects endogenous levels of total NET-5.
RRID:
AB_2841782
Cite Format: Affinity Biosciences Cat# DF8578, RRID:AB_2841782.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6720474K14Rik; 9430079M16Rik; AU018597; NET 5; NET5; New EST tetraspan 5; PP1057; Tetraspan NET 5; Tetraspan NET-5; Tetraspanin-9; Transmembrane 4 superfamily member tetraspan NET 5; TSN9_HUMAN; Tspan-9; TSPAN9;

Immunogens

Immunogen:
Uniprot:

O75954(TSN9_HUMAN) >>Visit HPA database.

Gene(ID):
TSPAN9(10867)
Expression:
O75954 TSN9_HUMAN:

Expressed in megakaryocytes and platelets (at protein level).

Sequence:
MARGCLCCLKYMMFLFNLIFWLCGCGLLGVGIWLSVSQGNFATFSPSFPSLSAANLVIAIGTIVMVTGFLGCLGAIKENKCLLLSFFIVLLVILLAELILLILFFVYMDKVNENAKKDLKEGLLLYHTENNVGLKNAWNIIQAEMRCCGVTDYTDWYPVLGENTVPDRCCMENSQGCGRNATTPLWRTGCYEKVKMWFDDNKHVLGTVGMCILIMQILGMAFSMTLFQHIHRTGKKYDA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
86
Chicken
86
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

PTMs:

Glycosylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.
Note: Colocalizes with GP6 in tetraspanin microdomains on the platelet surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in megakaryocytes and platelets (at protein level).

Subunit Structure:

Found in a complex with GP6.

Family&Domains:

Belongs to the tetraspanin (TM4SF) family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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