Phospho-Dab1 (Tyr232) Antibody | Affinity Biosciences

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Product:	Phospho-Dab1 (Tyr232) Antibody
Catalog:	AF3029
Description:	Rabbit polyclonal antibody to Phospho-Dab1 (Tyr232)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	60kDa; 64kD(Calculated).
Uniprot:	O75553
RRID:	AB_2834459

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

Phospho-Dab1 (Tyr232) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyrosine 232.

RRID:

AB_2834459
Cite Format: Affinity Biosciences Cat# AF3029, RRID:AB_2834459.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

Immunogens

Immunogen:

Uniprot:

O75553(DAB1_HUMAN) >>Visit HPA database.

Gene(ID):

DAB1(1600)

Expression:

O75553 DAB1_HUMAN:

Mainly expressed in brain.

Description:

DAB1 an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer.

Sequence:

O75553 DAB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75553 As Substrate

O75553

Site	PTM Type	Enzyme	Source
K43	Ubiquitination		Uniprot
K45	Ubiquitination		Uniprot
K84	Sumoylation		Uniprot
Y198	Phosphorylation		Uniprot
Y220	Phosphorylation		Uniprot
Y232	Phosphorylation		Uniprot
S433	Phosphorylation		Uniprot
T506	Phosphorylation		Uniprot
S524	Phosphorylation	P24941 (CDK2)	Uniprot
S526	Phosphorylation		Uniprot
S548	Phosphorylation		Uniprot

Research Backgrounds

O75553_DAB1_HUMAN

Function:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

PTMs:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

Tissue Specificity:

Mainly expressed in brain.

Subunit Structure:

Associates with the SH2 domains of SRC, FYN and ABL (By similarity). Interacts (phosphorylated on tyrosine residues) with CRK and CRKL (via respective SH2 domain) (By similarity). Interacts with SIAH1, LRP8 and VLDLR (By similarity). Interacts with LRP1. Interacts with APLP1 (via NPXY motif) (By similarity). Interacts with DAB2IP (By similarity).

Family&Domains:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

References

1). Ephrin‑b3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpine‑induced model of epilepsy. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018 (PubMed: 29512697) [IF=5.4]

Application: WB Species: rat Sample: hippocampus

Figure 3. |Reelin pathway expression in the hippocampus of rats during the development of SE.(E) Western blot analysis for p‑Dab1. β‑actin was used as an endogenous control. (F) Quantification of western blot results of p‑Dab1, presented as the p‑Dab1/Dab1 ratio. Data are presented as the mean ± standard deviation. ***P<0.0001, **P<0.001 by two‑way analysis of variance with Tukey's post hoc test (n=3 rats/per group). Ep, epilepsy; Con, control; Dab1, disabled 1; p‑Dab, phosphorylated Dab1.

Application: IHC Species: rat Sample: hippocampus

Figure 5. |Effect of EphB3‑Fc on reelin pathway expression in the hippocampus post‑SE.(C) Representative immunohistochemical staining of p‑Dab1 following EphB3‑Fc/Fc‑control treatment. Left: representative immunohistochemical staining of p‑Dab1 (scale bar=50 µm); right: high‑magnification bright‑field images and the arrows indicate the immunopositive cells (scale bar=10 µm).

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Phospho-Dab1 (Tyr232) Antibody - #AF3029