IL-20R alpha Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from Rat brain, using IL-20R alpha Antibody at 1/1000 dilution.

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using IL-20R alpha Antibody at 1/1000 dilution.

DF8565 staining Hela cells by IF/ICC.

Product:	IL-20R alpha Antibody
Catalog:	DF8565
Description:	Rabbit polyclonal antibody to IL-20R alpha
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Rat
Mol.Wt.:	62 kDa; 62kD(Calculated).
Uniprot:	Q9UHF4
RRID:	AB_2841769

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Rat(%)

Clonality:

Polyclonal

Specificity:

IL-20R alpha Antibody detects endogenous levels of total IL-20R alpha.

RRID:

AB_2841769
Cite Format: Affinity Biosciences Cat# DF8565, RRID:AB_2841769.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

class II cytokine receptor ZCYTOR7; CRF2 8; CRF2-8; Cytokine receptor class II member 8; Cytokine receptor class-II member 8; Cytokine receptor family 2 member 8; I20RA_HUMAN; IL 20R alpha; IL 20R1; IL-20 receptor subunit alpha; IL-20R-alpha; IL-20R1; IL-20RA; IL20RA; Interleukin 20 receptor alpha chain; Interleukin 20 receptor, alpha; interleukin-20 receptor I; Interleukin-20 receptor subunit alpha; ZcytoR7;

Immunogens

Immunogen:

Uniprot:

Q9UHF4(I20RA_HUMAN) >>Visit HPA database.

Gene(ID):

IL20RA(53832)

Expression:

Q9UHF4 I20RA_HUMAN:

Widely expressed with highest levels in skin and testis and high levels in brain. Highly expressed in psoriatic skin.

Sequence:

Q9UHF4 I20RA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRAPGRPALRPLPLPPLLLLLLAAPWGRAVPCVSGGLPKPANITFLSINMKNVLQWTPPEGLQGVKVTYTVQYFIYGQKKWLNKSECRNINRTYCDLSAETSDYEHQYYAKVKAIWGTKCSKWAESGRFYPFLETQIGPPEVALTTDEKSISVVLTAPEKWKRNPEDLPVSMQQIYSNLKYNVSVLNTKSNRTWSQCVTNHTLVLTWLEPNTLYCVHVESFVPGPPRRAQPSEKQCARTLKDQSSEFKAKIIFWYVLPVSITVFLFSVMGYSIYRYIHVGKEKHPANLILIYGNEFDKRFFVPAEKIVINFITLNISDDSKISHQDMSLLGKSSDVSSLNDPQPSGNLRPPQEEEEVKHLGYASHLMEIFCDSEENTEGTSLTQQESLSRTIPPDKTVIEYEYDVRTTDICAGPEEQELSLQEEVSTQGTLLESQAALAVLGPQTLQYSYTPQLQDLDPLAQEHTDSEEGPEEEPSTTLVDWDPQTGRLCIPSLSSFDQDSEGCEPSEGDGLGEEGLLSRLYEEPAPDRPPGENETYLMQFMEEWGLYVQMEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9UHF4_I20RA_HUMAN

Function:

The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL20RA/IL10RB dimer is a receptor for IL26.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Widely expressed with highest levels in skin and testis and high levels in brain. Highly expressed in psoriatic skin.

Subunit Structure:

Heterodimer with IL20RB and heterodimer with IL10RB.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IL-20R alpha Antibody - #DF8565