IDH3B Antibody - #DF8562

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Product: IDH3B Antibody
Catalog: DF8562
Description: Rabbit polyclonal antibody to IDH3B
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 42 kDa; 42kD(Calculated).
Uniprot: O43837
RRID: AB_2841766

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
IDH3B Antibody detects endogenous levels of total IDH3B.
RRID:
AB_2841766
Cite Format: Affinity Biosciences Cat# DF8562, RRID:AB_2841766.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ11043; H-IDHB; Idh3B; IDH3B_HUMAN; Isocitrate dehydrogenase [NAD] subunit beta; Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial; isocitrate dehydrogenase 3, beta subunit; Isocitric dehydrogenase; Isocitric dehydrogenase subunit beta; MGC903; mitochondrial; NAD(+)-specific ICDH; NAD(+)-specific ICDH subunit beta; NAD+-specific isocitrate dehydrogenase b subunit; NAD+-specific isocitrate dehydrogenase beta; OTTHUMP00000030023; OTTHUMP00000030024; RP46;

Immunogens

Immunogen:
Uniprot:

O43837(IDH3B_HUMAN) >>Visit HPA database.

Gene(ID):
IDH3B(3420)
Sequence:
MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
91
Bovine
73
Sheep
73
Rabbit
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43837 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
T11 Phosphorylation
K96 Acetylation
K96 Ubiquitination
K105 Ubiquitination
T117 Phosphorylation
Y121 Phosphorylation
K135 Ubiquitination
K146 Acetylation
T167 Phosphorylation
Y171 Phosphorylation
S172 Phosphorylation
S173 Phosphorylation
S178 Phosphorylation
K199 Acetylation
K199 Ubiquitination
K207 Acetylation
K212 Ubiquitination
K218 Acetylation
K218 Ubiquitination
K242 Ubiquitination
S330 Phosphorylation
K350 Acetylation
Y366 Phosphorylation
T368 Phosphorylation
T369 Phosphorylation
K374 Acetylation
S375 Phosphorylation
K383 Ubiquitination

Research Backgrounds

Function:

Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.

Family&Domains:

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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