IDH2 Antibody | Affinity Biosciences

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FIGURE 5 Verification of the differentially expressed proteins.

Product:	IDH2 Antibody
Catalog:	DF8561
Description:	Rabbit polyclonal antibody to IDH2
Application:	WB IF/ICC IHC-P
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	50 kDa; 51kD(Calculated).
Uniprot:	P48735
RRID:	AB_2841765

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

IDH2 Antibody detects endogenous levels of total IDH2.

RRID:

AB_2841765
Cite Format: Affinity Biosciences Cat# DF8561, RRID:AB_2841765.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D2HGA2; ICD-M; IDH; IDH2; IDHM; IDHP_HUMAN; IDP; IDPM; Isocitrate dehydrogenase [NADP], mitochondrial; Isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase;

Immunogens

Immunogen:

Uniprot:

P48735(IDHP_HUMAN) >>Visit HPA database.

Gene(ID):

IDH2(3418)

Sequence:

P48735 IDHP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48735 As Substrate

P48735

Site	PTM Type	Source
K48	Acetylation	Uniprot
K67	Acetylation	Uniprot
K69	Acetylation	Uniprot
K69	Ubiquitination	Uniprot
K80	Acetylation	Uniprot
K80	Ubiquitination	Uniprot
T97	Phosphorylation	Uniprot
S100	Phosphorylation	Uniprot
K106	Acetylation	Uniprot
K106	Ubiquitination	Uniprot
Y107	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
K112	Ubiquitination	Uniprot
T115	Phosphorylation	Uniprot
T117	Phosphorylation	Uniprot
K127	Acetylation	Uniprot
K127	Ubiquitination	Uniprot
K133	Acetylation	Uniprot
S134	Phosphorylation	Uniprot
T146	Phosphorylation	Uniprot
K155	Acetylation	Uniprot
K155	Ubiquitination	Uniprot
K166	Acetylation	Uniprot
K166	Ubiquitination	Uniprot
Y179	Phosphorylation	Uniprot
K180	Acetylation	Uniprot
K180	Ubiquitination	Uniprot
T182	Phosphorylation	Uniprot
R188	Methylation	Uniprot
T191	Phosphorylation	Uniprot
K193	Ubiquitination	Uniprot
T197	Phosphorylation	Uniprot
K199	Ubiquitination	Uniprot
K242	Acetylation	Uniprot
Y247	Phosphorylation	Uniprot
K256	Acetylation	Uniprot
K256	Ubiquitination	Uniprot
K263	Acetylation	Uniprot
K263	Ubiquitination	Uniprot
K272	Acetylation	Uniprot
K272	Ubiquitination	Uniprot
K275	Acetylation	Uniprot
K275	Ubiquitination	Uniprot
K280	Acetylation	Uniprot
K282	Acetylation	Uniprot
K282	Ubiquitination	Uniprot
S301	Phosphorylation	Uniprot
C308	S-Nitrosylation	Uniprot
T350	Phosphorylation	Uniprot
K384	Acetylation	Uniprot
K384	Ubiquitination	Uniprot
K400	Ubiquitination	Uniprot
K413	Acetylation	Uniprot
K413	Ubiquitination	Uniprot
S423	Phosphorylation	Uniprot
K426	Ubiquitination	Uniprot
K442	Acetylation	Uniprot
K442	Ubiquitination	Uniprot

Research Backgrounds

P48735_IDHP_HUMAN

Function:

Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

PTMs:

Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

Subcellular Location:

Mitochondrion.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. LIVER INTERNATIONAL, 2022 (PubMed: 34854209) [IF=6.7]

Application: WB Species: Human Sample: HepG2 cells

FIGURE 5 Verification of the differentially expressed proteins. A, the RT‐PCR detected the relative mRNA expression levels of AZGP1, GPC3, CTSF, NOTCH1, CTSD, uPAR, BMP2, LCN2, IDH2 and RPS3A (*P < .05). B, A representative western blot analysis for AZGP1, GPC3, NOTCH1, CTSD, uPAR, BMP2, LCN2, IDH2 and RPS3A expression in intracellular and extracellular samples from HepG2 cells. (Bars indicate SD, *P < .05)

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For Research Use Only.
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IDH2 Antibody - #DF8561