GnRH I Antibody - #DF8553

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Product: GnRH I Antibody
Catalog: DF8553
Description: Rabbit polyclonal antibody to GnRH I
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Monkey, Chicken, Xenopus
Mol.Wt.: 10 kDa; 10kD(Calculated).
Uniprot: P01148
RRID: AB_2841757

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Monkey(%), Chicken(83%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
GnRH I Antibody detects endogenous levels of total GnRH I.
RRID:
AB_2841757
Cite Format: Affinity Biosciences Cat# DF8553, RRID:AB_2841757.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GnRH associated peptide 1; GNRH1; Gonadotrophin releasing hormone 1; GRH; HH12; LHRH; LNRH; Luliberin I; Luteinizing releasing hormone; Lutenizing hormome releasing hormone; Progonadoliberin 1; Progonadoliberin I; Prolactin release inhibiting factor;

Immunogens

Immunogen:
Uniprot:

P01148(GON1_HUMAN) >>Visit HPA database.

Gene(ID):
GNRH1(2796)
Sequence:
MKPIQKLLAGLILLTWCVEGCSSQHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQPRSPLRDLKGALESLIEEETGQKKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the GnRH family.

References

1). The mechanism of Oatp1a5-mediated microcystin-leucine arginine entering into GnRH neurons. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2019 (PubMed: 31526925) [IF=6.8]

Application: WB    Species: mouse    Sample: GT1-7cells

Fig. 4. | N-linked glycosylation is involved in the process of Oatp1a5 transporting MC-LR. GT1-7 cells were pretreated with N-linked glycosylation inhibitor tunicamycin at a concentration of 0.01 μg/mL for 1 h, and followed by the treatment with MC-LR at a concentration of 500 nM for 24 h. (A) The presence of MC-LR in the cells was measured by immunofluorescence assay (scale bar = 50 μm). (B) Intracellular uptake of MC-LR by the transfected cells were detected by western blotting using an anti-MC-LR antibody. (C) The expression of GnRH was examined by western blotting.
2). Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021 (PubMed: 33396074) [IF=6.8]

Application: IHC    Species: mice    Sample: Brain

Fig. 2. MC-LR induced apoptosis of GnRH neurons. (A) Apoptotic cells in the hypothalamus of mice were detected by TUNEL test. The apoptotic cells with brown stained nuclei were stained by DAB, and apoptotic index was calculated based on the percentage of TUNEL positive cells in an image (scale bar = 50 µm, n = 6 mice/group, **P < 0.01 vs. the control). (B) A confocal fluorescence microscope was used to detect colocalization of Alexa Fluor 594-labled GPR54 (red), Alexa Fluor 488-labled active-caspase3 (green) and DAPI (blue) (scale bar = 50 µm, n = 3). The marker protein of GnRH neurons is GPR54.(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
3). Aspartame Intake Delayed Puberty Onset in Female Offspring Rats and Girls. Molecular nutrition & food research, 2024 (PubMed: 38389198) [IF=5.2]
4). OPN5 Regulating Mechanism of Follicle Development Through the TSH-DIO2/DIO3 Pathway in Mountain Ducks Under Different Photoperiods. Frontiers in Physiology, 2022 (PubMed: 35733985) [IF=4.0]
5). Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2021 (PubMed: 33340652) [IF=2.2]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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