GCS alpha-1 Antibody | Affinity Biosciences

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Product:	GCS alpha-1 Antibody
Catalog:	DF8551
Description:	Rabbit polyclonal antibody to GCS alpha-1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Dog
Mol.Wt.:	77 kDa; 77kD(Calculated).
Uniprot:	Q02108
RRID:	AB_2841755

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Dog(90%)

Clonality:

Polyclonal

Specificity:

GCS alpha-1 Antibody detects endogenous levels of total GCS alpha-1.

RRID:

AB_2841755
Cite Format: Affinity Biosciences Cat# DF8551, RRID:AB_2841755.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GC S alpha 1; GC SA3; GCS alpha 1; GCS alpha 3; GCS-alpha-1; GCS-alpha-3; GCYA3_HUMAN; Guanylate cyclase 1 soluble alpha 3; Guanylate cyclase soluble subunit alpha 1; Guanylate cyclase soluble subunit alpha 3; Guanylate cyclase soluble subunit alpha-3; Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit; Guc1a1; GUC1A3; GUCA3; GUCSA3; Gucy1a1; Gucy1a3; MYMY6; nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit; NOGC, alpha-1 subunit; Soluble guanylate cyclase large subunit;

Immunogens

Immunogen:

Uniprot:

Q02108(GCYA1_HUMAN) >>Visit HPA database.

Gene(ID):

GUCY1A3(2982)

Expression:

Q02108 GCYA1_HUMAN:

Detected in brain cortex and lung (at protein level).

Sequence:

Q02108 GCYA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02108 As Substrate

Q02108

Site	PTM Type	Source
S58	Phosphorylation	Uniprot
T64	Phosphorylation	Uniprot
T73	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
K133	Ubiquitination	Uniprot
S173	Phosphorylation	Uniprot
S174	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
K447	Ubiquitination	Uniprot
Y523	Phosphorylation	Uniprot
K606	Ubiquitination	Uniprot
K615	Ubiquitination	Uniprot
K627	Ubiquitination	Uniprot

Research Backgrounds

Q02108_GCYA1_HUMAN

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Detected in brain cortex and lung (at protein level).

Subunit Structure:

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1B1.

Family&Domains:

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

References

1). Effect of verapamil in the reversal of doxorubicin chemotherapy resistance in advanced gastric cancer. European Review for Medical and Pharmacological Sciences, 2020 (PubMed: 32744702) [IF=3.3]

Application: WB Species: human Sample: GC cells

Figure 2. |Evaluation of candidate gene/protein expression in GC cells. A, RT-qPCR detection of candidate gene expression in GC cells. Independent experiments were performed during the whole in vitro study. B, Western blot analysis of candidate protein expression levels in GC cells.

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GCS alpha-1 Antibody - #DF8551