GCLC Antibody - #DF8550

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Product: GCLC Antibody
Catalog: DF8550
Description: Rabbit polyclonal antibody to GCLC
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Pig
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 72 kDa; 73kD(Calculated).
Uniprot: P48506
RRID: AB_2841754

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GCLC Antibody detects endogenous levels of total GCLC.
RRID:
AB_2841754
Cite Format: Affinity Biosciences Cat# DF8550, RRID:AB_2841754.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 6.3.2.2; Gamma ECS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; Gclc; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN;

Immunogens

Immunogen:
Uniprot:

P48506(GSH1_HUMAN) >>Visit HPA database.

Gene(ID):
GCLC(2729)
Sequence:
MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48506 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S8 Phosphorylation
T15 Phosphorylation
K16 Acetylation
Y53 Phosphorylation
S70 Phosphorylation
K73 Ubiquitination
K81 Ubiquitination
K161 Ubiquitination
K209 Ubiquitination
K211 Ubiquitination
S215 Phosphorylation
T220 Phosphorylation
T222 Phosphorylation
K318 Ubiquitination
Y322 Phosphorylation
S334 Phosphorylation
K338 Ubiquitination
K342 Ubiquitination
Y343 Phosphorylation
T381 Phosphorylation
S395 Phosphorylation
K412 Ubiquitination
S464 Phosphorylation
K465 Ubiquitination
K492 Acetylation
Y587 Phosphorylation
S621 Phosphorylation

Research Backgrounds

Subunit Structure:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

Family&Domains:

Belongs to the glutamate--cysteine ligase type 3 family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL (PubMed: 33509464) [IF=8.1]
2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE (PubMed: 36150346) [IF=7.9]
3). Echinacoside alleviates hypobaric hypoxia-induced memory impairment in C57 mice. PHYTOTHERAPY RESEARCH (PubMed: 30768741) [IF=7.2]

Application: WB    Species: mouse    Sample: Hippocampi

FIGURE 7 |Effects of different doses of echinacoside (ECH) on the expressions of nuclear factor E2 p45‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and γ‐glutamyl cysteine synthetase (γ‐GCS) in the mice exposed to hypobaric hypoxia in protein level. Hippocampi of the mice were collected, and the protein levels of Nrf2 (cytoplasmic and nuclear), HO‐1, NQO1, and γ‐GCS were evaluated by Western blot. *p < 0.05, **p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus hypoxia (HYP)
4). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function (PubMed: 33625409) [IF=6.1]
5). The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery. Aging-US (PubMed: 32452828) [IF=5.2]

Application: WB    Species: human    Sample: HConFs

Figure 5. | S58 promotes antioxidant defense of TGF-β2-induced HConFs. (A) Analysis of antioxidant capacity of cells at day 14 after GFS. (B) Intracellular ROS variation, and (C) mitochondrial superoxide variation were examined by flow cytometry. (D) Antioxidant protein SOD1/2, γ-GCS and CAT levels analyzed by western blotting in TGF-β2-treated HConFs in the presence or absence of S58 (20 nM). (E) Relative antioxidant gene levels in HConFs preconditioned with TGF-β2 in the presence or absence of S58 (20 nM) for 12h. All data indicate the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001.
6). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY (PubMed: 28137608) [IF=4.3]

Application: WB    Species: rat    Sample: PC12 cells

7). Tetrahydrocurcumin and octahydrocurcumin, the primary and final hydrogenated metabolites of curcumin, possess superior hepatic-protective effect against acetaminophen-induced liver injury: Role of CYP2E1 and Keap1-Nrf2 pathway. FOOD AND CHEMICAL TOXICOLOGY (PubMed: 30423402) [IF=4.3]

Application: WB    Species: mouse    Sample: liver

Figure 9. |Differential effects of THC, OHC and CUR on NQO1 and HO-1 expressions in liver. (A)Densitometry analysis of mRNA level; (B) Representative protein expression bands; (C) Densitometry analysis of protein level. Data are presented as the mean ± SD (n = 3). ##p < 0.01 versus control group. *p < 0.05, **p < 0.01 versus APAP-treated group. ∆p < 0.05, ∆∆p < 0.01 versus treatment of CUR group.
8). Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. AUTOIMMUNITY (PubMed: 34431434) [IF=3.5]
9). Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells. Oxidative Medicine and Cellular Longevity (PubMed: 28105249)

Application: WB    Species: pig    Sample: IPEC-J2 Cells

Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells. (a–d) IPEC-J2 cells were incubated with OEO 10

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