Glucokinase Antibody - #DF8549
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| Product: | Glucokinase Antibody |
| Catalog: | DF8549 |
| Description: | Rabbit polyclonal antibody to Glucokinase |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 52 kDa; 52kD(Calculated). |
| Uniprot: | P35557 |
| RRID: | AB_2841753 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8549, RRID:AB_2841753.
Fold/Unfold
ATP:D-hexose 6-phosphotransferase; FGQTL3; GCK; GK; GLK; Glucokinase; Hexokinase D pancreatic isozyme; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HHF3; HK IV; HK4; HKIV; HXK4_HUMAN; HXKP; LGLK; MODY2;
Immunogens
- P35557 HXK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35557 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K161 | Sumoylation | Uniprot | |
| K169 | Sumoylation | Uniprot | |
| K346 | Sumoylation | Uniprot | |
| C371 | S-Nitrosylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| Y413 | Phosphorylation | Uniprot | |
| K414 | Sumoylation | Uniprot | |
| K458 | Sumoylation | Uniprot | |
| K459 | Sumoylation | Uniprot |
Research Backgrounds
Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6-phosphate for the synthesis of glycogen. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.
Cytoplasm. Nucleus. Mitochondrion.
Note: Under low glucose concentrations, GCK associates with GCKR and the inactive complex is recruited to the hepatocyte nucleus.
Monomer. Interacts with MIDN; the interaction occurs preferentially at low glucose levels and results in inhibition of hexokinase activity. Interacts with GCKR; leading to sequestration in the nucleus.
Belongs to the hexokinase family.
Research Fields
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Galactose metabolism.
· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Biosynthesis of other secondary metabolites > Neomycin, kanamycin and gentamicin biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
References
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