FucT-IV Antibody | Affinity Biosciences

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Western blot analysis of extracts from B16F10, using FucT-IV Antibody.

Product:	FucT-IV Antibody
Catalog:	DF8547
Description:	Rabbit polyclonal antibody to FucT-IV
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	45 kDa, 55 kDa; 59kD(Calculated).
Uniprot:	P22083
RRID:	AB_2841751

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

FucT-IV Antibody detects endogenous levels of total FucT-IV.

RRID:

AB_2841751
Cite Format: Affinity Biosciences Cat# DF8547, RRID:AB_2841751.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3-FAL; Alpha-3-fucosyltransferase; ELAM 1 ligand; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; FUC-TIV; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase; fucosyltransferase IV; fucT-IV; FUTIV; galactoside 3-L-fucosyltransferase; Leu M1; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1;

Immunogens

Immunogen:

Uniprot:

P22083(FUT4_HUMAN) >>Visit HPA database.

Gene(ID):

FUT4(2526)

Sequence:

P22083 FUT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRRLWGAARKPSGAGWEKEWAEAPQEAPGAWSGRLGPGRSGRKGRAVPGWASWPAHLALAARPARHLGGAGQGPRPLHSGTAPFHSRASGERQRRLEPQLQHESRCRSSTPADAWRAEAALPVRAMGAPWGSPTAAAGGRRGWRRGRGLPWTVCVLAAAGLTCTALITYACWGQLPPLPWASPTPSRPVGVLLWWEPFGGRDSAPRPPPDCRLRFNISGCRLLTDRASYGEAQAVLFHHRDLVKGPPDWPPPWGIQAHTAEEVDLRVLDYEEAAAAAEALATSSPRPPGQRWVWMNFESPSHSPGLRSLASNLFNWTLSYRADSDVFVPYGYLYPRSHPGDPPSGLAPPLSRKQGLVAWVVSHWDERQARVRYYHQLSQHVTVDVFGRGGPGQPVPEIGLLHTVARYKFYLAFENSQHLDYITEKLWRNALLAGAVPVVLGPDRANYERFVPRGAFIHVDDFPSASSLASYLLFLDRNPAVYRRYFHWRRSYAVHITSFWDEPWCRVCQAVQRAGDRPKSIRNLASWFER

PTMs - P22083 As Substrate

P22083

Site	PTM Type	Source
R75	Methylation	Uniprot
S79	Phosphorylation	Uniprot
R87	Methylation	Uniprot
R221	Methylation	Uniprot
R226	Methylation	Uniprot
S228	Phosphorylation	Uniprot
Y229	Phosphorylation	Uniprot
R240	Methylation	Uniprot
Y410	Phosphorylation	Uniprot

Research Backgrounds

P22083_FUT4_HUMAN

Function:

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Subcellular Location:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein.
Note: Membrane-bound form in trans cisternae of Golgi.

Family&Domains:

Belongs to the glycosyltransferase 10 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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FucT-IV Antibody - #DF8547