Endo G Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Endo G Antibody.

DF8541 at 1/100 staining Mouse lung tissue by IHC-P.

Figure 5.

Product:	Endo G Antibody
Catalog:	DF8541
Description:	Rabbit polyclonal antibody to Endo G
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep
Mol.Wt.:	32 kDa; 33kD(Calculated).
Uniprot:	Q14249
RRID:	AB_2841745

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Protocols

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

Endo G Antibody detects endogenous levels of total Endo G.

RRID:

AB_2841745
Cite Format: Affinity Biosciences Cat# DF8541, RRID:AB_2841745.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EndoG; Endonuclease G; Endonuclease G mitochondrial; EndonucleaseG; FLJ27463; Mitochondrial endonuclease G; NUCG_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q14249(NUCG_HUMAN) >>Visit HPA database.

Gene(ID):

ENDOG(2021)

Sequence:

Q14249 NUCG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14249 As Substrate

Q14249

Site	PTM Type	Source
T9	Phosphorylation	Uniprot
S12	Phosphorylation	Uniprot
K66	Ubiquitination	Uniprot
K76	Ubiquitination	Uniprot
K153	Ubiquitination	Uniprot
K181	Ubiquitination	Uniprot
K210	Ubiquitination	Uniprot
K214	Ubiquitination	Uniprot
K220	Ubiquitination	Uniprot
K290	Ubiquitination	Uniprot

Research Backgrounds

Q14249_NUCG_HUMAN

Function:

Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).

Subcellular Location:

Mitochondrion.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the DNA/RNA non-specific endonuclease family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

References

1). Akkermansia muciniphila Aspartic Protease Amuc_1434* Inhibits Human Colorectal Cancer LS174T Cell Viability via TRAIL-Mediated Apoptosis Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020 (PubMed: 32403433) [IF=5.6]

Application: WB Species: human Sample: LS174T cells

Figure 5. | Amuc_1434* mediated the activation of the apoptosis pathway in LS174T cells.(B) Amuc_1434* indirectly affected the expression of proteins in the mitochondrial apoptosis pathway.(a) B-cell lymphoma-2 interacting-domain death agonist (Bid), B-cell lymphoma-2-Associated X Protein(Bax), B-cell lymphoma-2 (Bcl-2), Cytochrome c (CytC), and Endonuclease G (EndoG) proteins of LS174T cells were detected using Western blot analysis after treatment with Amuc_1434* for 24 h.β-actin was used as the loading control.

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Endo G Antibody - #DF8541