Product: ENaC gamma Antibody
Catalog: DF8540
Description: Rabbit polyclonal antibody to ENaC gamma
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog
Mol.Wt.: 75-95 kDa; 74kD(Calculated).
Uniprot: P51170
RRID: AB_2841744

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(93%), Horse(83%), Dog(93%)
Clonality:
Polyclonal
Specificity:
ENaC gamma Antibody detects endogenous levels of total ENaC gamma.
RRID:
AB_2841744
Cite Format: Affinity Biosciences Cat# DF8540, RRID:AB_2841744.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amiloride sensitive epithelial sodium channel gamma subunit; Amiloride sensitive sodium channel subunit gamma; Amiloride-sensitive sodium channel subunit gamma; BESC3; ENaC gamma subunit; ENaCG; ENaCgamma; Epithelial Na(+) channel subunit gamma; Epithelial Na+ channel subunit gamma; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage gated sodium channel 1 subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; PHA1; SCNEG; SCNN 1G; SCNN1G; SCNNG_HUMAN; Sodium channel non voltage gated 1 gamma subunit; Sodium channel nonvoltage gated 1 gamma;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51170 SCNNG_HUMAN:

Expressed in kidney (at protein level).

Sequence:
MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYCLNTNTHGCRRIVVSRGRLRRLLWIGFTLTAVALILWQCALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQETREALKSLYGFPESRKRREAESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKRKVGGSIIHKASNVMHIESKQVVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWLLPVLTWDQGRQVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDDDLPTFNSALHLPPALGTQVPGTPPPKYNTLRLERAFSNQLTDTQMLDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
93
Dog
93
Horse
83
Sheep
78
Pig
72
Chicken
69
Rabbit
67
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51170 As Substrate

Site PTM Type Enzyme
Ubiquitination
T98 Phosphorylation
S108 Phosphorylation
T109 Phosphorylation
T176 Phosphorylation
K495 Acetylation
K498 Acetylation
K503 Acetylation
T622 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
T629 Phosphorylation
S637 Phosphorylation
T641 Phosphorylation

Research Backgrounds

Function:

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

PTMs:

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein.
Note: Apical membrane of epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney (at protein level).

Subunit Structure:

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4; via the WW domains. Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full-length immature form of PCSK9 (pro-PCSK9).

Family&Domains:

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

References

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience, 2022 (PubMed: 36419851) [IF=5.8]

2). Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway. Frontiers in Pharmacology, 2020 (PubMed: 33362546) [IF=5.6]

Application: WB    Species: mouse    Sample: lung tissues

FIGURE 4 | Melatonin enhanced ENaC expression in CLP-induced ALI. The protein expressions of α-ENaC (A), β-ENaC (B) and γ-ENaC (C) in ALI mouse were determined by western blot using GAPDH as a loading control. Results obtained by densitometry ratios of target proteins to GAPDH. The mRNA expressions of α-ENaC (D), β-ENaC (E) and γ-ENaC (F) in ALI mouse were tested by real-time PCR. Gene expression was normalized with GAPDH as the housekeeping gene, and the relative expression of genes was calculated using the 2−△△Ct method. (G) The distribution and protein expression of α-ENaC was examined by immunofluorescence. All values are means ± SD (n  6). ***p < 0.001; **p < 0.01; *p < 0.05 (one-way ANOVA followed by Turkey post hoc test).

3). Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway. HYPERTENSION RESEARCH, 2021 (PubMed: 32908237) [IF=5.4]

Application: WB    Species: rat    Sample: renal cortex and medulla

Fig. 4 |Effects of NaBu on DOCA/salt-induced renal sodium transporter proteins.Representative immunoblots of α-ENaC, β-ENaC, γ-ENaC,NHE3, NCC, and NKCC-2 and summarized intensities of blots in the renal cortex (a) and medulla (b) (n = 6–7 per group).αENaC α-subunit of epithelial sodium channel, β-ENaC βsubunit of epithelial sodium channel, γ-ENaC γ-subunit of epithelial sodium channel,NHE3 sodium/proton exchanger isoform 3, NKCC-2 Na-K-2Cl cotransporter, NCC Na-Cl Cotransporter

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.