ENaC gamma Antibody | Affinity Biosciences

IHC
Cites

1/4

DF8540 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

DF8540 at 1/100 staining Rat kidney tissue by IHC-P.

FIGURE 4 | Melatonin enhanced ENaC expression in CLP-induced ALI.

Fig.

Product:	ENaC gamma Antibody
Catalog:	DF8540
Description:	Rabbit polyclonal antibody to ENaC gamma
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Dog
Mol.Wt.:	75-95 kDa; 74kD(Calculated).
Uniprot:	P51170
RRID:	AB_2841744

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(93%), Horse(83%), Dog(93%)

Clonality:

Polyclonal

Specificity:

ENaC gamma Antibody detects endogenous levels of total ENaC gamma.

RRID:

AB_2841744
Cite Format: Affinity Biosciences Cat# DF8540, RRID:AB_2841744.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amiloride sensitive epithelial sodium channel gamma subunit; Amiloride sensitive sodium channel subunit gamma; Amiloride-sensitive sodium channel subunit gamma; BESC3; ENaC gamma subunit; ENaCG; ENaCgamma; Epithelial Na(+) channel subunit gamma; Epithelial Na+ channel subunit gamma; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage gated sodium channel 1 subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; PHA1; SCNEG; SCNN 1G; SCNN1G; SCNNG_HUMAN; Sodium channel non voltage gated 1 gamma subunit; Sodium channel nonvoltage gated 1 gamma;

Immunogens

Immunogen:

Uniprot:

P51170(SCNNG_HUMAN) >>Visit HPA database.

Gene(ID):

SCNN1G(6340)

Expression:

P51170 SCNNG_HUMAN:

Expressed in kidney (at protein level).

Sequence:

P51170 SCNNG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYCLNTNTHGCRRIVVSRGRLRRLLWIGFTLTAVALILWQCALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQETREALKSLYGFPESRKRREAESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKRKVGGSIIHKASNVMHIESKQVVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMHGNCYTFNNRENETILSTSMGGSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETAMVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWLLPVLTWDQGRQVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDDDLPTFNSALHLPPALGTQVPGTPPPKYNTLRLERAFSNQLTDTQMLDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Dog

Horse

Sheep

Pig

Chicken

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51170 As Substrate

P51170

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
T98	Phosphorylation		Uniprot
S108	Phosphorylation		Uniprot
T109	Phosphorylation		Uniprot
T176	Phosphorylation		Uniprot
K495	Acetylation		Uniprot
K498	Acetylation		Uniprot
K503	Acetylation		Uniprot
T622	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T629	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
T641	Phosphorylation		Uniprot

Research Backgrounds

P51170_SCNNG_HUMAN

Function:

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

PTMs:

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.

Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.

ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein.
Note: Apical membrane of epithelial cells.

Tissue Specificity:

Expressed in kidney (at protein level).

Subunit Structure:

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4; via the WW domains. Interacts with NEDD4L; via the WW domains. Interacts with WWP1; via the WW domains. Interacts with WWP2; via the WW domains. Interacts with the full-length immature form of PCSK9 (pro-PCSK9).

Family&Domains:

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

References

1). Renal lysophospholipase A1 contributes to Enterococcus faecalis-induced hypertension by enhancing sodium reabsorption. iScience, 2022 (PubMed: 36419851) [IF=5.8]

2). Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway. Frontiers in Pharmacology, 2020 (PubMed: 33362546) [IF=5.6]

Application: WB Species: mouse Sample: lung tissues

FIGURE 4 | Melatonin enhanced ENaC expression in CLP-induced ALI. The protein expressions of α-ENaC (A), β-ENaC (B) and γ-ENaC (C) in ALI mouse were determined by western blot using GAPDH as a loading control. Results obtained by densitometry ratios of target proteins to GAPDH. The mRNA expressions of α-ENaC (D), β-ENaC (E) and γ-ENaC (F) in ALI mouse were tested by real-time PCR. Gene expression was normalized with GAPDH as the housekeeping gene, and the relative expression of genes was calculated using the 2−△△Ct method. (G) The distribution and protein expression of α-ENaC was examined by immunofluorescence. All values are means ± SD (n 6). ***p < 0.001; **p < 0.01; *p < 0.05 (one-way ANOVA followed by Turkey post hoc test).

3). Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway. HYPERTENSION RESEARCH, 2021 (PubMed: 32908237) [IF=5.4]

Application: WB Species: rat Sample: renal cortex and medulla

Fig. 4 |Effects of NaBu on DOCA/salt-induced renal sodium transporter proteins.Representative immunoblots of α-ENaC, β-ENaC, γ-ENaC,NHE3, NCC, and NKCC-2 and summarized intensities of blots in the renal cortex (a) and medulla (b) (n = 6–7 per group).αENaC α-subunit of epithelial sodium channel, β-ENaC βsubunit of epithelial sodium channel, γ-ENaC γ-subunit of epithelial sodium channel,NHE3 sodium/proton exchanger isoform 3, NKCC-2 Na-K-2Cl cotransporter, NCC Na-Cl Cotransporter

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ENaC gamma Antibody - #DF8540