ECP Antibody - #DF8536

(3)  
Product: ECP Antibody
Catalog: DF8536
Description: Rabbit polyclonal antibody to ECP
Application: WB IHC
Reactivity: Human, Monkey
Mol.Wt.: 18 kDa; 18kD(Calculated).
Uniprot: P12724
RRID: AB_2841740

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Clonality:
Polyclonal
Specificity:
ECP Antibody detects endogenous levels of total ECP.
RRID:
AB_2841740
Cite Format: Affinity Biosciences Cat# DF8536, RRID:AB_2841740.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cytotoxic ribonuclease; ECP; ECP_HUMAN; Eosinophil cationic protein; OTTHUMP00000164017; Ribonuclease 3; Ribonuclease, RNase A family, 3; RNase 3; RNASE3; RNS3;

Immunogens

Immunogen:
Uniprot:

P12724(ECP_HUMAN) >>Visit HPA database.

Gene(ID):
RNASE3(6037)
Sequence:
MVPKLFTSQICLLLLLGLMGVEGSLHARPPQFTRAQWFAIQHISLNPPRCTIAMRAINNYRWRCKNQNTFLRTTFANVVNVCGNQSIRCPHNRTLNNCHRSRFRVPLLHCDLINPGAQNISNCTYADRPGRRFYVVACDNRDPRDSPRYPVVPVHLDTTI

PTMs - P12724 As Substrate

Site PTM Type Enzyme
Y149 Phosphorylation
T158 Phosphorylation
T159 Phosphorylation

Research Backgrounds

Function:

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Subcellular Location:

Secreted.
Note: Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). In vitro interacts with and insert into lipid bilayers composed of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol. In vitro, tends to form amyloid-like aggregates at pH 3, but not at pH 5, nor 7.

Family&Domains:

Belongs to the pancreatic ribonuclease family.

Research Fields

· Human Diseases > Immune diseases > Asthma.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.