EAAT1 Antibody - #DF8534
| Product: | EAAT1 Antibody |
| Catalog: | DF8534 |
| Description: | Rabbit polyclonal antibody to EAAT1 |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 59 kDa; 60kD(Calculated). |
| Uniprot: | P43003 |
| RRID: | AB_2841738 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8534, RRID:AB_2841738.
Fold/Unfold
EA6; EAA1_HUMAN; EAAT1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST-1; GLAST1; Glial high affinity glutamate transporter; glutamate/aspartate transporter, high affinity, sodium-dependent; High affinity neuronal glutamate transporter; Slc1a3; Sodium dependent glutamate/aspartate transporter; Sodium-dependent glutamate/aspartate transporter 1; Solute carrier family 1 (glial high affinity glutamate transporter) member 3; Solute carrier family 1 member 3;
Immunogens
Detected in brain (PubMed:8218410, PubMed:7521911, PubMed:8123008). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911).
- P43003 EAA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTKSNGEEPKMGGRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVTAVIVGTILGFTLRPYRMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPVGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGATINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43003 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| Y523 | Phosphorylation | Uniprot | |
| K534 | Ubiquitination | Uniprot | |
| K541 | Ubiquitination | Uniprot |
Research Backgrounds
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).
Glycosylated.
Cell membrane>Multi-pass membrane protein.
Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Homotrimer.
Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A3 subfamily.
Research Fields
· Organismal Systems > Nervous system > Glutamatergic synapse.
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