Defensin alpha1 Antibody - #DF8527

(1)   (1)  
Product: Defensin alpha1 Antibody
Catalog: DF8527
Description: Rabbit polyclonal antibody to Defensin alpha1
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 10 kDa; 10kD(Calculated).
Uniprot: P59665
RRID: AB_2841731

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Defensin alpha1 Antibody detects endogenous levels of total Defensin alpha1.
RRID:
AB_2841731
Cite Format: Affinity Biosciences Cat# DF8527, RRID:AB_2841731.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DEF1; DEF3; DEFA1; DEFA2; DEFA3; Defensin alpha 1; Defensin alpha 2; Defensin alpha 3; HNP1; HNP3; HP1; HP3; MRS; Neutrophil defensin 1; Neutrophil defensin 2; Neutrophil defensin 3;

Immunogens

Immunogen:
Uniprot:

P59665(DEF1_HUMAN) >>Visit HPA database.

Gene(ID):
DEFA1; DEFA1B(1667)
Sequence:
MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC

PTMs - P59665 As Substrate

Site PTM Type Enzyme
Y67 Phosphorylation
Y80 Phosphorylation
T82 Phosphorylation
C83 S-Nitrosylation
Y85 Phosphorylation

Research Backgrounds

Function:

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

PTMs:

ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production.

Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Dimer. Interacts with RETN.

Family&Domains:

Belongs to the alpha-defensin family.

References

1). Genetically Engineered Goats as Efficient Mammary Gland Bioreactors for Production of Recombinant Human Neutrophil Peptide 1 Using CRISPR/Cas9. Biology, 2024 (PubMed: 38927247) [IF=4.2]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.