Product: CDC34 Antibody
Catalog: DF8520
Description: Rabbit polyclonal antibody to CDC34
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 26 kDa; 27kD(Calculated).
Uniprot: P49427
RRID: AB_2841725

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
CDC34 Antibody detects endogenous levels of total CDC34.
RRID:
AB_2841725
Cite Format: Affinity Biosciences Cat# DF8520, RRID:AB_2841725.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cdc 34; Cdc34; Cell division cycle 34; Cell division cycle 34 homolog (S. cerevisiae); Cell division cycle 34 homolog; E2 CDC34; UB2R1_HUMAN; UBC 3; UBC3; UBCH3; UBE2 R1; UBE2R1; Ubiquitin carrier protein; Ubiquitin conjugating enzyme Cdc34; Ubiquitin conjugating enzyme E2 32 kDa complementing; Ubiquitin protein ligase; Ubiquitin protein ligase R1; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P49427 UB2R1_HUMAN:

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

Sequence:
MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
88
Rabbit
75
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49427 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K18 Ubiquitination
K63 Ubiquitination
Y68 Phosphorylation
S71 Phosphorylation
T89 Phosphorylation
K157 Ubiquitination
T162 Phosphorylation Q15418 (RPS6KA1)
R166 Methylation
K167 Ubiquitination
K182 Ubiquitination
Y190 Phosphorylation
K193 Ubiquitination
S203 Phosphorylation P68400 (CSNK2A1) , P67870 (CSNK2B)
S222 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
S231 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
T233 Phosphorylation P68400 (CSNK2A1) , P67870 (CSNK2B)
S236 Phosphorylation P68400 (CSNK2A1) , P67870 (CSNK2B)

Research Backgrounds

Function:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

PTMs:

Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.

Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.

Subcellular Location:

Cytoplasm. Nucleus.
Note: The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

Subunit Structure:

Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and RBX1. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. When phosphorylated, interacts with beta-TrCP (BTRC). Interacts with human herpes virus 1 protein ICP0 and associates with the proteasome for degradation. Interacts with casein kinase subunit CSNK2B.

Family&Domains:

The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.

Belongs to the ubiquitin-conjugating enzyme family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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