BRCAA1 Antibody - #DF8508

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Product: BRCAA1 Antibody
Catalog: DF8508
Description: Rabbit polyclonal antibody to BRCAA1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 147 kDa; 148kD(Calculated).
Uniprot: Q4LE39
RRID: AB_2841714

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(86%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BRCAA1 Antibody detects endogenous levels of total BRCAA1.
RRID:
AB_2841714
Cite Format: Affinity Biosciences Cat# DF8508, RRID:AB_2841714.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARID 4B; ARID domain-containing protein 4B; ARID4B; AT rich interactive domain 4B (Rbp1 like); AT rich interactive domain 4B; BCAA; BRCAA 1; BRCAA1; Breast cancer associated antigen 1; Breast cancer associated antigen BRCAA 1; Breast cancer associated antigen BRCAA1; Breast carcinoma associated antigen; DKFZp313M2420; Histone deacetylase complex subunit SAP180; MGC163290; Rb binding protein homolog; RBBP1 L1; RBBP1L1; Rbp 1 like; Rbp1 l1; Rbp1 like; RBP1 like protein; RBP1L; Rbp1l1; Retinoblastoma binding protein 1 like 1; SAP 180; SAP180; SIN3A associated protein 180;

Immunogens

Immunogen:
Uniprot:

Q4LE39(ARI4B_HUMAN) >>Visit HPA database.

Gene(ID):
ARID4B(51742)
Expression:
Q4LE39 ARI4B_HUMAN:

Highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Sequence:
MKALDEPPYLTVGTDVSAKYRGAFCEAKIKTAKRLVKVKVTFRHDSSTVEVQDDHIKGPLKVGAIVEVKNLDGAYQEAVINKLTDASWYTVVFDDGDEKTLRRSSLCLKGERHFAESETLDQLPLTNPEHFGTPVIGKKTNRGRRSNHIPEEESSSSSSDEDEDDRKQIDELLGKVVCVDYISLDKKKALWFPALVVCPDCSDEIAVKKDNILVRSFKDGKFTSVPRKDVHEITSDTAPKPDAVLKQAFEQALEFHKSRTIPANWKTELKEDSSSSEAEEEEEEEDDEKEKEDNSSEEEEEIEPFPEERENFLQQLYKFMEDRGTPINKRPVLGYRNLNLFKLFRLVHKLGGFDNIESGAVWKQVYQDLGIPVLNSAAGYNVKCAYKKYLYGFEEYCRSANIEFQMALPEKVVNKQCKECENVKEIKVKEENETEIKEIKMEEERNIIPREEKPIEDEIERKENIKPSLGSKKNLLESIPTHSDQEKEVNIKKPEDNENLDDKDDDTTRVDESLNIKVEAEEEKAKSGDETNKEEDEDDEEAEEEEEEEEEEEDEDDDDNNEEEEFECYPPGMKVQVRYGRGKNQKMYEASIKDSDVEGGEVLYLVHYCGWNVRYDEWIKADKIVRPADKNVPKIKHRKKIKNKLDKEKDKDEKYSPKNCKLRRLSKPPFQTNPSPEMVSKLDLTDAKNSDTAHIKSIEITSILNGLQASESSAEDSEQEDERGAQDMDNNGKEESKIDHLTNNRNDLISKEEQNSSSLLEENKVHADLVISKPVSKSPERLRKDIEVLSEDTDYEEDEVTKKRKDVKKDTTDKSSKPQIKRGKRRYCNTEECLKTGSPGKKEEKAKNKESLCMENSSNSSSDEDEEETKAKMTPTKKYNGLEEKRKSLRTTGFYSGFSEVAEKRIKLLNNSDERLQNSRAKDRKDVWSSIQGQWPKKTLKELFSDSDTEAAASPPHPAPEEGVAEESLQTVAEEESCSPSVELEKPPPVNVDSKPIEEKTVEVNDRKAEFPSSGSNSVLNTPPTTPESPSSVTVTEGSRQQSSVTVSEPLAPNQEEVRSIKSETDSTIEVDSVAGELQDLQSEGNSSPAGFDASVSSSSSNQPEPEHPEKACTGQKRVKDAQGGGSSSKKQKRSHKATVVNNKKKGKGTNSSDSEELSAGESITKSQPVKSVSTGMKSHSTKSPARTQSPGKCGKNGDKDPDLKEPSNRLPKVYKWSFQMSDLENMTSAERITILQEKLQEIRKHYLSLKSEVASIDRRRKRLKKKERESAATSSSSSSPSSSSITAAVMLTLAEPSMSSASQNGMSVECR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
86
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q4LE39 As Substrate

Site PTM Type Enzyme
K28 Ubiquitination
K57 Ubiquitination
Y89 Phosphorylation
T90 Phosphorylation
S105 Phosphorylation
T126 Phosphorylation
T133 Phosphorylation
K257 Ubiquitination
S273 Phosphorylation
S275 Phosphorylation
S276 Phosphorylation
S295 Phosphorylation
S296 Phosphorylation
Y335 Phosphorylation
K429 Sumoylation
K440 Sumoylation
K466 Ubiquitination
S471 Phosphorylation
S483 Phosphorylation
T507 Phosphorylation
T508 Phosphorylation
K647 Acetylation
K649 Acetylation
K654 Acetylation
Y655 Phosphorylation
S656 Phosphorylation
S666 Phosphorylation
T672 Phosphorylation
S675 Phosphorylation
K688 Ubiquitination
S717 Phosphorylation
K751 Ubiquitination
S758 Phosphorylation
S776 Phosphorylation
S778 Phosphorylation
S790 Phosphorylation
T793 Phosphorylation
Y795 Phosphorylation
Y827 Phosphorylation
T830 Phosphorylation
T836 Phosphorylation
S838 Phosphorylation
T874 Phosphorylation
K878 Acetylation
S888 Phosphorylation
Y895 Phosphorylation
S896 Phosphorylation
S899 Phosphorylation
K904 Acetylation
K904 Ubiquitination
S912 Phosphorylation
S919 Phosphorylation
S930 Phosphorylation
S1014 Phosphorylation
T1022 Phosphorylation
T1025 Phosphorylation
T1026 Phosphorylation
S1029 Phosphorylation
S1031 Phosphorylation
S1032 Phosphorylation
T1036 Phosphorylation
S1060 Phosphorylation
K1131 Acetylation
T1150 Phosphorylation
S1152 Phosphorylation
S1153 Phosphorylation
S1155 Phosphorylation
S1159 Phosphorylation
S1174 Phosphorylation
K1178 Acetylation
K1183 Acetylation
S1184 Phosphorylation
S1190 Phosphorylation
K1245 Ubiquitination
S1256 Phosphorylation

Research Backgrounds

Function:

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic in breast cancer cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Subunit Structure:

Component of a Sin3A corepressor complex consisting of SIN3A, SAP130, SUDS3/SAP45, SAP180, HDAC1 and HDAC2. Interacts with ARID4A. Interacts with AR (By similarity).

Family&Domains:

The C-terminus mediates interaction with mSin3A corepressor complex.

The N-terminus is involved in transcriptional repression by HDAC-independent mechanisms.

The ARID domain is involved in stabilizing the mSin3A corepressor complex on DNA.

Restrictive clause

 

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