ATP7A Antibody | Affinity Biosciences

IF/ICC
Cites

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Figure 7 Expression of the ATP7A and ATP7B proteins in MD and WD fibroblasts.

Product:	ATP7A Antibody
Catalog:	DF8506
Description:	Rabbit polyclonal antibody to ATP7A
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	163 kDa; 163kD(Calculated).
Uniprot:	Q04656
RRID:	AB_2841712

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(87%), Horse(93%), Sheep(87%), Rabbit(100%), Dog(93%)

Clonality:

Polyclonal

Specificity:

ATP7A Antibody detects endogenous levels of total ATP7A.

RRID:

AB_2841712
Cite Format: Affinity Biosciences Cat# DF8506, RRID:AB_2841712.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP 7A; ATP7A; ATP7A_HUMAN; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); ATPase Cu++ transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1; Copper-transporting ATPase 1; Cu++ transporting P type ATPase; DSMAX; FLJ17790; MC 1; MC1; Menkes disease associated protein; Menkes disease-associated protein; Menkes syndrome; MK; MNK; OHS; OTTHUMP00000062077; SMAX3;

Immunogens

Immunogen:

Uniprot:

Q04656(ATP7A_HUMAN) >>Visit HPA database.

Gene(ID):

ATP7A(538)

Expression:

Q04656 ATP7A_HUMAN:

Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.

Sequence:

Q04656 ATP7A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTATFIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEASLVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLATTIAFAYSLIILLVAMYERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Dog

Bovine

Sheep

Zebrafish

Xenopus

Pig

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04656 As Substrate

Q04656

Site	PTM Type	Source
S147	Phosphorylation	Uniprot
T152	Phosphorylation	Uniprot
K155	Ubiquitination	Uniprot
K195	Ubiquitination	Uniprot
K226	Acetylation	Uniprot
K227	Acetylation	Uniprot
K239	Acetylation	Uniprot
Y244	Phosphorylation	Uniprot
K246	Ubiquitination	Uniprot
S266	Phosphorylation	Uniprot
S270	Phosphorylation	Uniprot
S272	Phosphorylation	Uniprot
Y273	Phosphorylation	Uniprot
T278	Phosphorylation	Uniprot
Y321	Phosphorylation	Uniprot
S325	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot
S330	Phosphorylation	Uniprot
K333	Ubiquitination	Uniprot
S339	Phosphorylation	Uniprot
Y343	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
S353	Phosphorylation	Uniprot
T354	Phosphorylation	Uniprot
S355	Phosphorylation	Uniprot
S357	Phosphorylation	Uniprot
S359	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
S362	Phosphorylation	Uniprot
Y471	Phosphorylation	Uniprot
T476	Phosphorylation	Uniprot
Y517	Phosphorylation	Uniprot
S518	Phosphorylation	Uniprot
Y594	Phosphorylation	Uniprot
T601	Phosphorylation	Uniprot
K633	Ubiquitination	Uniprot
S827	Phosphorylation	Uniprot
K849	Ubiquitination	Uniprot
K855	Ubiquitination	Uniprot
K1037	Ubiquitination	Uniprot
K1039	Ubiquitination	Uniprot
K1045	Ubiquitination	Uniprot
K1060	Ubiquitination	Uniprot
K1132	Ubiquitination	Uniprot
T1204	Phosphorylation	Uniprot
T1212	Phosphorylation	Uniprot
S1306	Phosphorylation	Uniprot
K1412	Ubiquitination	Uniprot
S1424	Phosphorylation	Uniprot
S1430	Phosphorylation	Uniprot
S1432	Phosphorylation	Uniprot
S1435	Phosphorylation	Uniprot
S1444	Phosphorylation	Uniprot
S1447	Phosphorylation	Uniprot
K1449	Ubiquitination	Uniprot
Y1459	Phosphorylation	Uniprot
S1460	Phosphorylation	Uniprot
S1463	Phosphorylation	Uniprot
S1466	Phosphorylation	Uniprot
S1469	Phosphorylation	Uniprot
K1471	Ubiquitination	Uniprot
S1473	Phosphorylation	Uniprot
S1476	Phosphorylation	Uniprot
T1479	Phosphorylation	Uniprot
S1480	Phosphorylation	Uniprot
S1486	Phosphorylation	Uniprot
T1498	Phosphorylation	Uniprot

Research Backgrounds

Q04656_ATP7A_HUMAN

Function:

May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane (PubMed:9147644). Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.

Cytoplasm>Cytosol.

Endoplasmic reticulum.

Tissue Specificity:

Subunit Structure:

Monomer. Interacts with PDZD11. Interacts with ATOX1 and COMMD1.

Family&Domains:

The C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Metformin-based carbon dots based on biguanide functional groups for simultaneous chelation of copper ions and inhibitable colorectal cancer therapy. Carbon, 2023 [IF=10.9]

2). Copper transporter gene ATP7A: A predictive biomarker for immunotherapy and targeted therapy in hepatocellular carcinoma. International immunopharmacology, 2023 (PubMed: 36502594) [IF=5.6]

Application: IHC Species: Human Sample: HCC tissues

Fig. 5. ATP7A is associated with CD3 + T cells and CD8 + T cells in HCC. (A) Immunohistochemical analysis of ATP7A and CD3, CD8 expression in HCC tissues. (B-E) Association between ATP7A expression and CD3, CD8 expression in HCC patients. ***, P < 0.001.

3). Response of Fibroblasts from Menkes' and Wilson's Copper Metabolism-Related Disorders to Ionizing Radiation: Influence of the Nucleo-Shuttling of the ATM Protein Kinase. Biomolecules, 2023 (PubMed: 38136617) [IF=5.5]

Application: WB Species: Human Sample: fibroblasts

Figure 7 Expression of the ATP7A and ATP7B proteins in MD and WD fibroblasts. Anti-ATP7A and anti-ATP7B immunoblots with total or cytoplasmic protein extracts were applied to the indicated non-irradiated control Hs27, MD (A,B), and WD (C,D) fibroblasts. The grey levels corresponding to each condition are shown in Figure S4. Original western blot images can be found in raw data.

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ATP7A Antibody - #DF8506