Product: HSPA6 Antibody
Catalog: DF8465
Description: Rabbit polyclonal antibody to HSPA6
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse
Mol.Wt.: 71 kDa; 71kD(Calculated).
Uniprot: P17066
RRID: AB_2841698

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(82%), Horse(100%)
Clonality:
Polyclonal
Specificity:
HSPA6 Antibody detects endogenous levels of total HSPA6.
RRID:
AB_2841698
Cite Format: Affinity Biosciences Cat# DF8465, RRID:AB_2841698.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heat shock 70 kDa protein 6; Heat shock 70 kDa protein B'; Heat shock 70 kDa protein B''; heat shock 70kD protein 6 (HSP70B'); Heat shock 70kDa protein 6; HSP70B'; HSP70B-Prime; HSP76_HUMAN; HSPA6; OTTHUMP00000032372;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MQAPRELAVGIDLGTTYSCVGVFQQGRVEILANDQGNRTTPSYVAFTDTERLVGDAAKSQAALNPHNTVFDAKRLIGRKFADTTVQSDMKHWPFRVVSEGGKPKVRVCYRGEDKTFYPEEISSMVLSKMKETAEAYLGQPVKHAVITVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDRRGAGERNVLIFDLGGGTFDVSVLSIDAGVFEVKATAGDTHLGGEDFDNRLVNHFMEEFRRKHGKDLSGNKRALRRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVFIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILSVTATDRSTGKANKITITNDKGRLSKEEVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIPEEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYGGPGVPGGSSCGTQARQGDPSTGPIIEEVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
82
Bovine
82
Sheep
73
Rabbit
70
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17066 As Substrate

Site PTM Type Enzyme
T39 Phosphorylation
T40 Phosphorylation
S42 Phosphorylation
Y43 Phosphorylation
T47 Phosphorylation
T49 Phosphorylation
R51 Methylation
K58 Ubiquitination
S59 Phosphorylation
K73 Ubiquitination
K79 Ubiquitination
K90 Ubiquitination
K102 Ubiquitination
K104 Ubiquitination
K114 Ubiquitination
S123 Phosphorylation
K128 Ubiquitination
K130 Ubiquitination
K142 Methylation
K142 Ubiquitination
K161 Ubiquitination
Y185 Phosphorylation
T224 Phosphorylation
K259 Ubiquitination
T267 Phosphorylation
S278 Phosphorylation
S298 Phosphorylation
T300 Phosphorylation
S309 Phosphorylation
S314 Phosphorylation
T315 Phosphorylation
K321 Acetylation
K321 Ubiquitination
K327 Ubiquitination
K330 Methylation
K330 Ubiquitination
K347 Ubiquitination
K350 Ubiquitination
K359 Acetylation
K359 Sumoylation
K359 Ubiquitination
K363 Sumoylation
K363 Ubiquitination
S364 Phosphorylation
Y373 Phosphorylation
K386 Ubiquitination
S402 Phosphorylation
T407 Phosphorylation
K453 Ubiquitination
R460 Methylation
R471 Methylation
R495 Methylation
K499 Ubiquitination
K502 Acetylation
K502 Ubiquitination
T504 Phosphorylation
K509 Acetylation
K509 Sumoylation
K509 Ubiquitination
K514 Ubiquitination
K528 Ubiquitination
K541 Ubiquitination
K552 Ubiquitination
K563 Methylation
K563 Ubiquitination
K598 Ubiquitination

Research Backgrounds

Function:

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.

Family&Domains:

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.

Belongs to the heat shock protein 70 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Genetic Information Processing > Transcription > Spliceosome.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). HSPA6 is Correlated With the Malignant Progression and Immune Microenvironment of Gliomas. Frontiers in Cell and Developmental Biology, 2022 (PubMed: 35281087) [IF=5.5]

Application: WB    Species: Human    Sample: glioma tissues and matched para-carcinoma tissues

FIGURE 4 Expression verification of HSPA6 in clinical brain tissues and different glioma cell lines and experimental validation of HSPA6 in U251 and U118 cells. (A) Western blot showing HSPA6 expression in six paired glioma tissues and matched para-carcinoma tissues from the same patient. HSPA6 protein expression levels were quantified by ImageJ software. (B) Western blot of HSPA6 expression in different glioma cell lines. HSPA6 protein expression levels were quantified by ImageJ software. (C) Cell scratch test to detect the migration ability of U251 and U118 cells which had been transfected with Vector-NC or Vector-HSPA6 and the sh-NC or sh-HSPA6 plasmids. The width of the wound was photographed under a microscope (magnification, 100x). (D) Cell Counting Kit-8 (CCK-8) assays were used to detect the proliferation of U251 and U118 cells which had been transfected with Vector-NC or Vector-HSPA6 and the sh-NC or sh-HSPA6 plasmids. (E) Transwell assays of U251 and U118 cells which had been transfected with Vector-NC or Vector-HSPA6 and sh-NC or sh-HSPA6 plasmids. Representative photographs (magnification, 200×) of migratory or invading cells on the membrane coated with or without Matrigel. Quantitative analysis of Transwell assays was performed by ImageJ software. (F) Flow cytometry detection of apoptosis in U251 and U118 cells which had been transfected with Vector-NC or Vector-HSPA6 and sh-NC or sh-HSPA6 plasmids. The proportion of apoptotic cells was equal to the sum of the proportion of early and late apoptosis. Data are expressed as the mean ± SEM from three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.

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