LMAN1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of LMAN1 using mouse brain lysates.

Product:	LMAN1 Antibody
Catalog:	DF8460
Description:	Rabbit polyclonal antibody to LMAN1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	58 kDa; 58kD(Calculated).
Uniprot:	P49257
RRID:	AB_2841696

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Zebrafish(83%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

LMAN1 Antibody detects endogenous levels of total LMAN1.

RRID:

AB_2841696
Cite Format: Affinity Biosciences Cat# DF8460, RRID:AB_2841696.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Endoplasmic reticulum golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; ERGIC-53; ERGIC53; ERGIC53 like protein; F5F8D; FMFD1; Gp58; Intracellular mannose specific lectin; Intracellular mannose-specific lectin MR60; Lectin mannose binding 1; Lectin mannose-binding 1; Lman1; LMAN1 like protein; LMAN1_HUMAN; MCFD1; MR60; Protein ERGIC-53;

Immunogens

Immunogen:

Uniprot:

P49257(LMAN1_HUMAN) >>Visit HPA database.

Gene(ID):

LMAN1(3998)

Expression:

P49257 LMAN1_HUMAN:

Ubiquitous.

Sequence:

P49257 LMAN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGSADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQNTLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSMSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVHFIIFVVVQTVLFIGYIMYRSQQEAAAKKFF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Sheep

Chicken

Zebrafish

Xenopus

Bovine

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49257 As Substrate

P49257

Site	PTM Type	Source
K49	Ubiquitination	Uniprot
K87	Ubiquitination	Uniprot
T276	O-Glycosylation	Uniprot
K302	Ubiquitination	Uniprot
S325	Phosphorylation	Uniprot
K346	Ubiquitination	Uniprot
K372	Ubiquitination	Uniprot
T385	O-Glycosylation	Uniprot
K407	Ubiquitination	Uniprot
S425	O-Glycosylation	Uniprot
S425	Phosphorylation	Uniprot
Y430	Phosphorylation	Uniprot
T432	O-Glycosylation	Uniprot
T433	O-Glycosylation	Uniprot
K440	Ubiquitination	Uniprot
K447	Ubiquitination	Uniprot

Research Backgrounds

P49257_LMAN1_HUMAN

Function:

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

PTMs:

The N-terminal may be partly blocked.

Subcellular Location:

Endoplasmic reticulum-Golgi intermediate compartment membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass membrane protein. Endoplasmic reticulum membrane>Single-pass type I membrane protein.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Exists both as a covalent disulfide-linked homohexamer, and a complex of three disulfide-linked dimers non-covalently kept together. Interacts with MCFD2. May interact with TMEM115. Interacts with RAB3GAP1 and RAB3GAP2. Interacts with UBXN6.

Family&Domains:

The FF ER export motif at the C-terminus is not sufficient to support endoplasmic reticulum exit, and needs assistance of Gln-501 for proper recognition of COPII coat components.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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LMAN1 Antibody - #DF8460