Product: DLG1 Antibody
Catalog: DF8454
Description: Rabbit polyclonal antibody to DLG1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 100 kDa; 100kD(Calculated).
Uniprot: Q12959
RRID: AB_2841693

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
DLG1 Antibody detects endogenous levels of total DLG1.
RRID:
AB_2841693
Cite Format: Affinity Biosciences Cat# DF8454, RRID:AB_2841693.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Discs large homolog 1; discs large, Drosophila, homolog of, 1; discs, large homolog 1 (Drosophila); Disks large homolog 1; dJ1061C18.1.1; DKFZp761P0818; DKFZp781B0426; DLG 1; DLG1; DLG1_HUMAN; DLGH 1; DLGH1; hDlg; Presynaptic protein SAP97; SAP 97; SAP-97; SAP97; Synapse associated protein 97; Synapse-associated protein 97;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q12959 DLG1_HUMAN:

Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Expressed in the brain (at protein level) (PubMed:23676497). Widely expressed, with isoforms displaying different expression profiles.

Sequence:
MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCIDRSKPSEPIQPVNTWEISSLPSSTVTSETLPSSLSPSVEKYRYQDEDTPPQEHISPQITNEVIGPELVHVSEKNLSEIENVHGFVSHSHISPIKPTEAVLPSPPTVPVIPVLPVPAENTVILPTIPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEVDVRDVTHSKAVEALKEAGSIVRLYVKRRKPVSEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNNVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYMNDGYAPPDITNSSSQPVDNHVSPSSFLGQTPASPARYSPVSKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSISSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRDKGEIPDDMGSKGLKHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVREVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTEEQARKTFERAMKLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGSYIWVPAKEKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12959 As Substrate

Site PTM Type Enzyme
Y18 Phosphorylation
S23 Phosphorylation
Y56 Phosphorylation
S102 Phosphorylation
S104 Phosphorylation
K107 Ubiquitination
Y110 Phosphorylation
T115 Phosphorylation
S122 Phosphorylation P53778 (MAPK12)
T126 Phosphorylation
S138 Phosphorylation
K140 Ubiquitination
S153 Phosphorylation
S155 Phosphorylation
S158 Phosphorylation P06493 (CDK1) , Q13164 (MAPK7) , P24941 (CDK2) , P53778 (MAPK12)
T209 Phosphorylation P53778 (MAPK12)
Y222 Phosphorylation
S232 Phosphorylation Q9UQM7 (CAMK2A)
K291 Ubiquitination
S301 Phosphorylation
K321 Ubiquitination
T351 Phosphorylation
K361 Ubiquitination
K370 Ubiquitination
Y399 Phosphorylation
K401 Ubiquitination
Y409 Phosphorylation
Y414 Phosphorylation
S432 Phosphorylation
S443 Phosphorylation P06493 (CDK1) , P53778 (MAPK12) , P24941 (CDK2)
Y447 Phosphorylation P53778 (MAPK12)
S448 Phosphorylation
S451 Phosphorylation
K452 Ubiquitination
S517 Phosphorylation
K556 Ubiquitination
S567 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S575 Phosphorylation
T578 Phosphorylation
S579 Phosphorylation
S583 Phosphorylation
Y585 Phosphorylation
S598 Phosphorylation
S602 Phosphorylation
S619 Phosphorylation
T630 Phosphorylation
S635 Phosphorylation
K644 Acetylation
K644 Ubiquitination
T657 Phosphorylation P17252 (PRKCA)
K659 Ubiquitination
S662 Phosphorylation
K677 Acetylation
T683 Phosphorylation
S684 Phosphorylation
S687 Phosphorylation
K724 Ubiquitination
Y753 Phosphorylation
Y760 Phosphorylation
T764 Phosphorylation
S765 Phosphorylation
Y784 Phosphorylation
Y789 Phosphorylation
T791 Phosphorylation
Y824 Phosphorylation
K843 Ubiquitination
T853 Phosphorylation
S892 Phosphorylation
Y895 Phosphorylation
K901 Ubiquitination

Research Backgrounds

Function:

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane.

PTMs:

Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A (By similarity).

Subcellular Location:

Membrane>Peripheral membrane protein. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Cell membrane>Sarcolemma. Apical cell membrane. Cell junction. Cytoplasm.
Note: Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells (PubMed:12807908). May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Expressed in the brain (at protein level). Widely expressed, with isoforms displaying different expression profiles.

Subunit Structure:

Homotetramer (Probable). Interacts (via guanylate kinase-like domain) with DLGAP1, DLGAP2, DLGAP3, DLGAP4 and MAP1A (By similarity). Interacts (via guanylate kinase-like domain) with KIF13B. May interact with HTR2A (By similarity). Interacts (via PDZ domains) with GRIA1 (By similarity). Interacts (via PDZ domains) with GRIN2A (By similarity). Interacts (via PDZ domains) with KCND2 and KCND3. Interacts (via PDZ domains) with KCNA1, KCNA2, KCNA3 and KCNA4. Interacts (via PDZ domains) with ADGRA3. Interacts with KCNF1. Interacts with CAMK2 (By similarity). Interacts with cytoskeleton-associated protein EPB41. Interacts with cytoskeleton-associated protein EZR (By similarity). Found in a complex with KCNA5 and CAV3 (By similarity). Found in a complex with APC and CTNNB1. Interacts with CDH1 through binding to PIK3R1. Forms multiprotein complexes with CASK, LIN7A, LIN7B, LIN7C, APBA1, and KCNJ12 (By similarity). Interacts with TOPK. Forms a tripartite complex composed of DLG1, MPP7 and LIN7 (LIN7A or LIN7C). May interact with TJAP1. Interacts with PTEN (By similarity). Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts with SFPQ. Interacts (via PDZ domains) with ADGRA2 (via PDZ-binding motif). Interacts with ADAM10; this interaction recruits ADAM10 to the cell membrane during long-term depression in hippocampal neurons.

(Microbial infection) Interacts with HTLV-1 protein Tax.

(Microbial infection) Interacts with human papillomavirus 18/HPV-18 protein E6.

Family&Domains:

The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.

The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.

The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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