MGEA5 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using MGEA5 Antibody.

Product:	MGEA5 Antibody
Catalog:	DF8436
Description:	Rabbit polyclonal antibody to MGEA5
Application:	WB IHC IF/ICC
Reactivity:	Human, Monkey
Mol.Wt.:	130 kDa; 103kD(Calculated).
Uniprot:	O60502
RRID:	AB_2841682

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Clonality:

Polyclonal

Specificity:

MGEA5 Antibody detects endogenous levels of total MGEA5.

RRID:

AB_2841682
Cite Format: Affinity Biosciences Cat# DF8436, RRID:AB_2841682.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta-hexosaminidase; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Bifunctional protein NCOAT; HEXC; Hyaluronidase in meningioma; KIAA0679; MEA5; Meningioma expressed antigen 5; Meningioma-expressed antigen 5; MGEA 5; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; Nuclear cytoplasmic O GlcNAcase and acetyltransferase; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; OGA_HUMAN; Protein O-GlcNAcase;

Immunogens

Immunogen:

Uniprot:

O60502(OGA_HUMAN) >>Visit HPA database.

Gene(ID):

MGEA5(10724)

Expression:

O60502 OGA_HUMAN:

Ubiquitous. Shows highest expression in the brain, placenta and pancreas.

Sequence:

O60502 OGA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYGRPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPGLDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPETFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSEDSTVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

PTMs - O60502 As Substrate

O60502

Site	PTM Type	Enzyme	Source
S6	Phosphorylation		Uniprot
K87	Ubiquitination		Uniprot
K98	Ubiquitination		Uniprot
K157	Ubiquitination		Uniprot
K240	Ubiquitination		Uniprot
K253	Ubiquitination		Uniprot
K257	Ubiquitination		Uniprot
S268	Phosphorylation		Uniprot
K269	Ubiquitination		Uniprot
K272	Ubiquitination		Uniprot
K289	Ubiquitination		Uniprot
K297	Ubiquitination		Uniprot
K358	Sumoylation		Uniprot
S364	Phosphorylation		Uniprot
T370	Phosphorylation		Uniprot
S405	O-Glycosylation		Uniprot
S505	Phosphorylation		Uniprot
S509	Phosphorylation		Uniprot
S511	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
K597	Ubiquitination		Uniprot
K599	Ubiquitination		Uniprot
K613	Ubiquitination		Uniprot
T709	Phosphorylation	P28482 (MAPK1)	Uniprot
S712	Phosphorylation		Uniprot
K723	Ubiquitination		Uniprot
K730	Ubiquitination		Uniprot
K794	Ubiquitination		Uniprot
K807	Ubiquitination		Uniprot
K850	Ubiquitination		Uniprot
K858	Ubiquitination		Uniprot
K869	Ubiquitination		Uniprot
K885	Ubiquitination		Uniprot
K893	Ubiquitination		Uniprot
C896	S-Nitrosylation		Uniprot
K901	Ubiquitination		Uniprot
K907	Ubiquitination		Uniprot
S915	Phosphorylation		Uniprot

Research Backgrounds

O60502_OGA_HUMAN

Function:

Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

PTMs:

Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.

Subcellular Location:

Nucleus.

Cytoplasm.

Tissue Specificity:

Ubiquitous. Shows highest expression in the brain, placenta and pancreas.

Subunit Structure:

Monomer. Interacts with CLOCK (By similarity).

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein Tax; this interaction increases Tax interacting partner CREB1 O-GlcNAcylation.

Family&Domains:

Belongs to the glycosyl hydrolase 84 family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

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MGEA5 Antibody - #DF8436