DOCK2 Antibody - #DF8431

(2)  
Product: DOCK2 Antibody
Catalog: DF8431
Description: Rabbit polyclonal antibody to DOCK2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 212 kDa; 212kD(Calculated).
Uniprot: Q92608
RRID: AB_2841679

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
DOCK2 Antibody detects endogenous levels of total DOCK2.
RRID:
AB_2841679
Cite Format: Affinity Biosciences Cat# DF8431, RRID:AB_2841679.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dedicator of cyto kinesis 2; Dedicator of cytokinesis 2; Dedicator of cytokinesis protein 2; DOCK 2; FLJ46592; KIAA0209;

Immunogens

Immunogen:
Uniprot:

Q92608(DOCK2_HUMAN) >>Visit HPA database.

Gene(ID):
DOCK2(1794)
Expression:
Q92608 DOCK2_HUMAN:

Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon.

Sequence:
MAPWRKADKERHGVAIYNFQGSGAPQLSLQIGDVVRIQETCGDWYRGYLIKHKMLQGIFPKSFIHIKEVTVEKRRNTENIIPAEIPLAQEVTTTLWEWGSIWKQLYVASKKERFLQVQSMMYDLMEWRSQLLSGTLPKDELKELKQKVTSKIDYGNKILELDLIVRDEDGNILDPDNTSVISLFHAHEEATDKITERIKEEMSKDQPDYAMYSRISSSPTHSLYVFVRNFVCRIGEDAELFMSLYDPNKQTVISENYLVRWGSRGFPKEIEMLNNLKVVFTDLGNKDLNRDKIYLICQIVRVGKMDLKDTGAKKCTQGLRRPFGVAVMDITDIIKGKAESDEEKQHFIPFHPVTAENDFLHSLLGKVIASKGDSGGQGLWVTMKMLVGDIIQIRKDYPHLVDRTTVVARKLGFPEIIMPGDVRNDIYITLLQGDFDKYNKTTQRNVEVIMCVCAEDGKTLPNAICVGAGDKPMNEYRSVVYYQVKQPRWMETVKVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGTTLHDGFHDLVVLKGDSKKMEDASAYLTLPSYRHHVENKGATLSRSSSSVGGLSVSSRDVFSISTLVCSTKLTQNVGLLGLLKWRMKPQLLQENLEKLKIVDGEEVVKFLQDTLDALFNIMMEHSQSDEYDILVFDALIYIIGLIADRKFQHFNTVLEAYIQQHFSATLAYKKLMTVLKTYLDTSSRGEQCEPILRTLKALEYVFKFIVRSRTLFSQLYEGKEQMEFEESMRRLFESINNLMKSQYKTTILLQVAALKYIPSVLHDVEMVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMNEIVQSNLFKKQECRDILLPVITKELKELLEQKDDMQHQVLERKYCVELLNSILEVLSYQDAAFTYHHIQEIMVQLLRTVNRTVITMGRDHILISHFVACMTAILNQMGDQHYSFYIETFQTSSELVDFLMETFIMFKDLIGKNVYPGDWMAMSMVQNRVFLRAINKFAETMNQKFLEHTNFEFQLWNNYFHLAVAFITQDSLQLEQFSHAKYNKILNKYGDMRRLIGFSIRDMWYKLGQNKICFIPGMVGPILEMTLIPEAELRKATIPIFFDMMLCEYQRSGDFKKFENEIILKLDHEVEGGRGDEQYMQLLESILMECAAEHPTIAKSVENFVNLVKGLLEKLLDYRGVMTDESKDNRMSCTVNLLNFYKDNNREEMYIRYLYKLRDLHLDCDNYTEAAYTLLLHTWLLKWSDEQCASQVMQTGQQHPQTHRQLKETLYETIIGYFDKGKMWEEAISLCKELAEQYEMEIFDYELLSQNLIQQAKFYESIMKILRPKPDYFAVGYYGQGFPSFLRNKVFIYRGKEYERREDFQMQLMTQFPNAEKMNTTSAPGDDVKNAPGQYIQCFTVQPVLDEHPRFKNKPVPDQIINFYKSNYVQRFHYSRPVRRGTVDPENEFASMWIERTSFVTAYKLPGILRWFEVVHMSQTTISPLENAIETMSTANEKILMMINQYQSDETLPINPLSMLLNGIVDPAVMGGFAKYEKAFFTEEYVRDHPEDQDKLTHLKDLIAWQIPFLGAGIKIHEKRVSDNLRPFHDRMEECFKNLKMKVEKEYGVREMPDFDDRRVGRPRSMLRSYRQMSIISLASMNSDCSTPSKPTSESFDLELASPKTPRVEQEEPISPGSTLPEVKLRRSKKRTKRSSVVFADEKAAAESDLKRLSRKHEFMSDTNLSEHAAIPLKASVLSQMSFASQSMPTIPALALSVAGIPGLDEANTSPRLSQTFLQLSDGDKKTLTRKKVNQFFKTMLASKSAEEGKQIPDSLSTDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
86
Xenopus
73
Sheep
40
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92608 As Substrate

Site PTM Type Enzyme
Y48 Phosphorylation
K51 Ubiquitination
K53 Ubiquitination
K61 Ubiquitination
K67 Acetylation
K67 Ubiquitination
K110 Ubiquitination
K138 Ubiquitination
K145 Acetylation
K151 Acetylation
K151 Ubiquitination
K199 Ubiquitination
S203 Phosphorylation
K204 Ubiquitination
Y209 Phosphorylation
Y212 Phosphorylation
S213 Phosphorylation
S218 Phosphorylation
S222 Phosphorylation
K268 Ubiquitination
K277 Ubiquitination
T281 Phosphorylation
K286 Ubiquitination
K304 Acetylation
K304 Ubiquitination
K308 Ubiquitination
T331 Phosphorylation
K337 Ubiquitination
K344 Ubiquitination
K366 Ubiquitination
K371 Ubiquitination
K395 Ubiquitination
K410 Ubiquitination
K437 Ubiquitination
K440 Ubiquitination
K471 Ubiquitination
Y481 Phosphorylation
Y482 Phosphorylation
K485 Ubiquitination
K494 Ubiquitination
K525 Ubiquitination
K533 Ubiquitination
K536 Ubiquitination
K553 Ubiquitination
K557 Methylation
K558 Ubiquitination
K578 Ubiquitination
S585 Phosphorylation
S586 Phosphorylation
S587 Phosphorylation
S588 Phosphorylation
S593 Phosphorylation
K610 Ubiquitination
T612 Phosphorylation
K622 Ubiquitination
K626 Ubiquitination
K636 Ubiquitination
K638 Ubiquitination
Y699 Phosphorylation
K712 Ubiquitination
K738 Acetylation
S755 Phosphorylation
Y758 Phosphorylation
Y798 Phosphorylation
S801 Phosphorylation
K835 Ubiquitination
S838 Phosphorylation
K849 Ubiquitination
K850 Ubiquitination
K863 Ubiquitination
K866 Ubiquitination
K872 Ubiquitination
K1006 Ubiquitination
K1054 Ubiquitination
K1058 Acetylation
K1058 Ubiquitination
K1076 Ubiquitination
Y1119 Phosphorylation
K1127 Ubiquitination
K1135 Ubiquitination
T1166 Phosphorylation
S1170 Phosphorylation
K1179 Ubiquitination
K1184 Ubiquitination
K1197 Ubiquitination
Y1220 Phosphorylation
K1226 Ubiquitination
S1260 Phosphorylation
K1290 Ubiquitination
K1292 Ubiquitination
S1354 Phosphorylation
K1359 Ubiquitination
K1387 Ubiquitination
T1390 Phosphorylation
T1391 Phosphorylation
S1392 Phosphorylation
K1399 Ubiquitination
K1422 Ubiquitination
K1424 Ubiquitination
Y1434 Phosphorylation
K1435 Ubiquitination
S1436 Phosphorylation
K1474 Ubiquitination
S1488 Phosphorylation
K1548 Ubiquitination
Y1555 Phosphorylation
K1565 Ubiquitination
K1570 Ubiquitination
S1592 Phosphorylation
R1601 Methylation
K1607 Ubiquitination
K1612 Ubiquitination
K1615 Ubiquitination
Y1617 Phosphorylation
S1635 Phosphorylation
S1639 Phosphorylation
S1644 Phosphorylation
T1657 Phosphorylation
S1672 Phosphorylation
T1675 Phosphorylation
S1685 Phosphorylation
S1688 Phosphorylation
T1689 Phosphorylation
K1694 Ubiquitination
S1698 Phosphorylation
T1702 Phosphorylation
S1705 Phosphorylation
S1706 Phosphorylation
K1713 Ubiquitination
K1721 Ubiquitination
S1724 Phosphorylation
K1726 Ubiquitination
S1731 Phosphorylation
T1733 Phosphorylation
S1736 Phosphorylation
S1757 Phosphorylation
S1780 Phosphorylation
S1784 Phosphorylation
T1786 Phosphorylation
S1791 Phosphorylation
K1795 Ubiquitination
K1796 Ubiquitination
T1797 Phosphorylation
T1799 Phosphorylation
K1802 Ubiquitination
K1808 Ubiquitination
T1809 Phosphorylation
K1814 Ubiquitination
K1820 Ubiquitination

Research Backgrounds

Function:

Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.

Subcellular Location:

Endomembrane system>Peripheral membrane protein. Cytoplasm>Cytoskeleton.
Note: Colocalizes with F-actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon.

Subunit Structure:

Homodimer (Probable). Interacts with RAC1 and RAC2. Interacts with CRKL and VAV. Interacts with CD3Z.

Family&Domains:

The DHR-2 domain may mediate the GEF activity.

Belongs to the DOCK family.

Research Fields

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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