Product: EIF3B Antibody
Catalog: DF8429
Description: Rabbit polyclonal antibody to EIF3B
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 116 kDa; 92kD(Calculated).
Uniprot: P55884
RRID: AB_2841677

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
EIF3B Antibody detects endogenous levels of total EIF3B.
RRID:
AB_2841677
Cite Format: Affinity Biosciences Cat# DF8429, RRID:AB_2841677.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

eIF-3-eta; eIF3 eta; eIF3 p110; eIF3 p116; EIF3-ETA; EIF3-P110; EIF3-P116; eIF3b; EIF3B_HUMAN; EIF3S9; EIF3S9, formerly; Eukaryotic translation initiation factor 3, subunit B; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit 9 eta; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit 9, formerly; hPrt1; Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of; PRT1; Prt1 homolog;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55884 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T68 Phosphorylation
S78 Phosphorylation
S81 Phosphorylation
S83 Phosphorylation
S85 Phosphorylation
S95 Phosphorylation
S117 Phosphorylation
S119 Phosphorylation
S125 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation
S164 Phosphorylation
K202 Ubiquitination
K204 Ubiquitination
K209 Acetylation
K209 Ubiquitination
K213 Acetylation
K213 Ubiquitination
K216 Ubiquitination
T218 Phosphorylation
K228 Ubiquitination
T229 Phosphorylation
K230 Sumoylation
K230 Ubiquitination
Y232 Phosphorylation
Y237 Phosphorylation
S239 Phosphorylation
K248 Ubiquitination
Y253 Phosphorylation
K254 Ubiquitination
K257 Ubiquitination
T267 Phosphorylation
Y272 Phosphorylation
K284 Ubiquitination
K288 Acetylation
K288 Ubiquitination
S312 Phosphorylation
K324 Ubiquitination
Y339 Phosphorylation
K345 Ubiquitination
K364 Acetylation
K364 Ubiquitination
T390 Phosphorylation
S392 Phosphorylation
T397 Phosphorylation
R416 Methylation
K436 Acetylation
S447 Phosphorylation
Y449 Phosphorylation
K459 Ubiquitination
K460 Ubiquitination
S461 Phosphorylation
K463 Ubiquitination
S465 Phosphorylation
S473 Phosphorylation
K487 Ubiquitination
T500 Phosphorylation
K516 Ubiquitination
K521 Ubiquitination
Y525 Phosphorylation
K529 Ubiquitination
K535 Ubiquitination
T537 Phosphorylation
K552 Ubiquitination
S575 Phosphorylation
K599 Ubiquitination
K604 Ubiquitination
K608 Ubiquitination
K693 Ubiquitination
T710 Phosphorylation
S713 Phosphorylation
K718 Ubiquitination
K726 Ubiquitination
S728 Phosphorylation
K729 Acetylation
K729 Ubiquitination
K734 Ubiquitination
S738 Phosphorylation
K741 Ubiquitination
K744 Acetylation
K744 Ubiquitination
T752 Phosphorylation
R758 Methylation
K762 Ubiquitination
Y768 Phosphorylation
K772 Ubiquitination

Research Backgrounds

Function:

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

PTMs:

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. Interacts with METTL3.

Family&Domains:

The RRM domain mediates interaction with EIF3J.

Belongs to the eIF-3 subunit B family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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