PIK3R4 Antibody - #DF8424

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Product: PIK3R4 Antibody
Catalog: DF8424
Description: Rabbit polyclonal antibody to PIK3R4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 153 kDa; 153kD(Calculated).
Uniprot: Q99570
RRID: AB_2841672

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(83%), Bovine(92%), Horse(100%), Rabbit(92%), Dog(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
PIK3R4 Antibody detects endogenous levels of total PIK3R4.
RRID:
AB_2841672
Cite Format: Affinity Biosciences Cat# DF8424, RRID:AB_2841672.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC102700; P150; Phosphatidylinositol 3 kinase associated p150; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3 kinase adaptor protein; Phosphoinositide 3 kinase regulatory subunit 4; Phosphoinositide 3 kinase regulatory subunit 4 p150; Phosphoinositide 3 kinase regulatory subunit polypeptide 4; Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3-kinase adaptor protein; Phosphoinositide 3-kinase regulatory subunit 4; PI3 kinase p150 subunit; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; PI3R4_HUMAN; PIK3R 4; PIK3R4; VPS 15; VPS15;

Immunogens

Immunogen:
Uniprot:

Q99570(PI3R4_HUMAN) >>Visit HPA database.

Gene(ID):
PIK3R4(30849)
Expression:
Q99570 PI3R4_HUMAN:

Ubiquitously expressed.

Sequence:
MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
92
Bovine
92
Dog
92
Rabbit
92
Xenopus
83
Zebrafish
83
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99570 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
T61 Phosphorylation
K68 Ubiquitination
K75 Ubiquitination
K89 Ubiquitination
K97 Ubiquitination
S115 Phosphorylation
T116 Phosphorylation
S169 Phosphorylation
T220 Phosphorylation
K280 Ubiquitination
K300 Ubiquitination
K309 Ubiquitination
S338 Phosphorylation
K348 Ubiquitination
K443 Ubiquitination
K449 Ubiquitination
K553 Ubiquitination
Y673 Phosphorylation
T680 Phosphorylation
K713 Ubiquitination
K736 Ubiquitination
S740 Phosphorylation
K772 Ubiquitination
K773 Ubiquitination
T780 Phosphorylation
K786 Ubiquitination
K791 Ubiquitination
K801 Ubiquitination
S808 Phosphorylation
S813 Phosphorylation
S814 Phosphorylation
K816 Ubiquitination
K835 Ubiquitination
K850 Ubiquitination
S853 Phosphorylation
S861 Phosphorylation
S865 Phosphorylation
K888 Ubiquitination
S892 Phosphorylation
S894 Phosphorylation
K918 Ubiquitination
S925 Phosphorylation
S926 Phosphorylation
T927 Phosphorylation
S931 Phosphorylation
K941 Ubiquitination
T942 Phosphorylation
K951 Acetylation
K951 Ubiquitination
K962 Ubiquitination
K973 Ubiquitination
K993 Ubiquitination
K1018 Ubiquitination
Y1038 Phosphorylation
S1074 Phosphorylation
S1079 Phosphorylation
K1081 Ubiquitination
Y1272 Phosphorylation
S1282 Phosphorylation
T1283 Phosphorylation
S1287 Phosphorylation
S1289 Phosphorylation
Y1290 Phosphorylation
Y1291 Phosphorylation
K1293 Ubiquitination
K1307 Ubiquitination
K1309 Ubiquitination
T1316 Phosphorylation

Research Backgrounds

Function:

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.

PTMs:

Myristoylated.

Probably autophosphorylated.

Subcellular Location:

Late endosome. Cytoplasmic vesicle>Autophagosome. Membrane>Lipid-anchor.
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2. PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34. Interacts with NRBF2.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

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