SRD5A2 Antibody - #DF8416

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Product: SRD5A2 Antibody
Catalog: DF8416
Description: Rabbit polyclonal antibody to SRD5A2
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: P31213
RRID: AB_2841665

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%)
Clonality:
Polyclonal
Specificity:
SRD5A2 Antibody detects endogenous levels of total SRD5A2.
RRID:
AB_2841665
Cite Format: Affinity Biosciences Cat# DF8416, RRID:AB_2841665.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 oxo 5 alpha steroid 4 dehydrogenase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha SR2; 5 alpha-SR2; 5ART2; e II 5 alpha reductase; EC; MGC138457; Micropenis, included; S5A2_HUMAN; S5AR 2; SR type 2; SRD5A2; Steroid 5 alpha reductase 2; Steroid 5-alpha-reductase 2; Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2); Type II 5-alpha reductase;

Immunogens

Immunogen:
Uniprot:

P31213(S5A2_HUMAN) >>Visit HPA database.

Gene(ID):
SRD5A2(6716)
Expression:
P31213 S5A2_HUMAN:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

Sequence:
MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
78
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31213 As Substrate

Site PTM Type Enzyme
Y91 Phosphorylation
Y98 Phosphorylation
Y165 Phosphorylation
S177 Phosphorylation

Research Backgrounds

Function:

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Subcellular Location:

Microsome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

Family&Domains:

Belongs to the steroid 5-alpha reductase family.

Research Fields

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

References

1). Risk subtyping and prognostic assessment of prostate cancer based on consensus genes. Communications Biology, 2022 (PubMed: 35293897) [IF=5.9]

Application: IHC    Species: Human    Sample: PCa tumor tissues

Fig. 4 Immunohistochemistry validation of SRD5A2 and ITGA11 expression in PCa tumor tissues. a Representative pictures showing the different protein levels of SRD5A2 in prostate cancer patients with different Gleason scores. Quantitative comparisons of H-scores were also performed in Gleason and pathological tumor stage subgroups. b Representative pictures showing the different protein levels of ITGA11 in prostate cancer patients with different Gleason scores. Quantitative comparison of staining density was also performed in Gleason and pathological tumor stage subgroups. Data are presented as the mean ± SD by t-test, bars represent mean values, error bars represent SD. Scale bars, 50 μm, 200 μm.

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