MYH2 Antibody - #DF8414

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Product: MYH2 Antibody
Catalog: DF8414
Description: Rabbit polyclonal antibody to MYH2
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 223 kDa; 223kD(Calculated).
Uniprot: Q9UKX2
RRID: AB_2841664

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
MYH2 Antibody detects endogenous levels of total MYH2.
RRID:
AB_2841664
Cite Format: Affinity Biosciences Cat# DF8414, RRID:AB_2841664.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

adult 2; Fast 2a myosin heavy chain; IBM3; Inclusion body myopathy 3, autosomal dominant; MYH2; MYH2_HUMAN; MYH2A; MYHas8; MyHC IIa; MyHC-2a; MyHC-IIa; MYHSA2; Myosin heavy chain 2; Myosin heavy chain 2a; Myosin heavy chain; Myosin heavy chain IIa; Myosin heavy chain skeletal muscle adult 2; Myosin heavy polypeptide 2 skeletal muscle adult; Myosin-2; MYPOP; skeletal muscle; Type IIA myosin heavy chain;

Immunogens

Immunogen:
Uniprot:

Q9UKX2(MYH2_HUMAN) >>Visit HPA database.

Gene(ID):
MYH2(4620)
Sequence:
MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATLTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEITSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVEQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDILQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKX2 As Substrate

Site PTM Type Enzyme
T51 Phosphorylation
S54 Phosphorylation
T69 Phosphorylation
Y163 Phosphorylation
T168 Phosphorylation
Y195 Phosphorylation
T198 Phosphorylation
T218 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
S263 Phosphorylation
Y269 Phosphorylation
K273 Ubiquitination
T381 Phosphorylation
Y389 Phosphorylation
Y404 Phosphorylation
Y413 Phosphorylation
T415 Phosphorylation
K416 Acetylation
K505 Acetylation
Y556 Phosphorylation
T607 Phosphorylation
Y612 Phosphorylation
S625 Phosphorylation
T629 Phosphorylation
T666 Phosphorylation
Y721 Phosphorylation
S734 Phosphorylation
S744 Phosphorylation
K746 Acetylation
S748 Phosphorylation
T792 Phosphorylation
Y822 Phosphorylation
K837 Acetylation
K847 Acetylation
K877 Acetylation
K924 Acetylation
S954 Phosphorylation
K957 Acetylation
K958 Acetylation
T966 Phosphorylation
T985 Phosphorylation
T994 Phosphorylation
T999 Phosphorylation
T1025 Phosphorylation
S1043 Phosphorylation
K1048 Acetylation
S1071 Phosphorylation
S1094 Phosphorylation
K1112 Acetylation
R1132 Methylation
S1134 Phosphorylation
S1143 Phosphorylation
S1146 Phosphorylation
T1163 Phosphorylation
S1164 Phosphorylation
T1188 Phosphorylation
T1194 Phosphorylation
T1197 Phosphorylation
S1205 Phosphorylation
S1228 Phosphorylation
S1239 Phosphorylation
T1243 Phosphorylation
S1245 Phosphorylation
S1263 Phosphorylation
S1267 Phosphorylation
T1280 Phosphorylation
T1288 Phosphorylation
S1294 Phosphorylation
S1305 Phosphorylation
S1308 Phosphorylation
T1315 Phosphorylation
S1341 Phosphorylation
Y1353 Phosphorylation
S1359 Phosphorylation
S1368 Phosphorylation
T1372 Phosphorylation
T1379 Phosphorylation
Y1381 Phosphorylation
T1383 Phosphorylation
T1389 Phosphorylation
K1396 Acetylation
K1397 Acetylation
K1398 Acetylation
T1423 Phosphorylation
K1450 Acetylation
S1482 Phosphorylation
T1485 Phosphorylation
Y1494 Phosphorylation
T1503 Phosphorylation
S1516 Phosphorylation
T1519 Phosphorylation
S1576 Phosphorylation
S1605 Phosphorylation
T1606 Phosphorylation
S1613 Phosphorylation
Y1649 Phosphorylation
T1652 Phosphorylation
S1669 Phosphorylation
S1704 Phosphorylation
S1716 Phosphorylation
T1724 Phosphorylation
T1727 Phosphorylation
S1728 Phosphorylation
T1732 Phosphorylation
K1733 Acetylation
S1741 Phosphorylation
T1766 Phosphorylation
K1776 Acetylation
K1790 Acetylation
K1797 Acetylation
S1834 Phosphorylation
K1837 Acetylation
T1857 Phosphorylation
Y1858 Phosphorylation
K1865 Acetylation
S1895 Phosphorylation
T1897 Phosphorylation
S1921 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Muscle contraction. Required for cytoskeleton organization (By similarity).

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with GCSAM.

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

References

1). Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ). Animals, 2020 (PubMed: 31963250) [IF=3.0]

Application: WB    Species: cattle    Sample: beef cattle

Figure 3. |The relative expression levels of CSRP3 (A) and MYH2 (B) proteins between tenderloin and flank steaks in beef cattle. β-actin was used as an internal reference protein.

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