NUP98 Antibody - #DF8408

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Product: NUP98 Antibody
Catalog: DF8408
Description: Rabbit polyclonal antibody to NUP98
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 188 kDa; 198kD(Calculated).
Uniprot: P52948
RRID: AB_2841659

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
NUP98 Antibody detects endogenous levels of total NUP98.
RRID:
AB_2841659
Cite Format: Affinity Biosciences Cat# DF8408, RRID:AB_2841659.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98 Nup96; Nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; Nup98; Nup98-Nup96; NUP98_HUMAN;

Immunogens

Immunogen:
Uniprot:

P52948(NUP98_HUMAN) >>Visit HPA database.

Gene(ID):
NUP98(4928)
Sequence:
MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAGTTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNTSGNSIFGSKPAPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFSFLSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMRAALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVVVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVLDTMLEESMPEDQEPVSASTHIASSLGINPHVLQIMKASLLTDEEDVDMALDQRFSRLPSKADTSQEICSPRLPISASHSSKTRSLVGGLLQSKFTSGAFLSPSVSVQECRTPRAASLMNIPSTSSWSVPPPLTSVFTMPSPAPEVPLKTVGTRRQLGLVPREKSVTYGKGKLLMDMALFMGRSFRVGWGPNWTLANSGEQLNGSHELENHQIADSMEFGFLPNPVAVKPLTESPFKVHLEKLSLRQRKPDEDMKLYQTPLELKLKHSTVHVDELCPLIVPNLGVAVIHDYADWVKEASGDLPEAQIVKHWSLTWTLCEALWGHLKELDSQLNEPREYIQILERRRAFSRWLSCTATPQIEEEVSLTQKNSPVEAVFSYLTGKRISEACSLAQQSGDHRLALLLSQFVGSQSVRELLTMQLVDWHQLQADSFIQDERLRIFALLAGKPVWQLSEKKQINVCSQLDWKRSLAIHLWYLLPPTASISRALSMYEEAFQNTSDSDRYACSPLPSYLEGSGCVIAEEQNSQTPLRDVCFHLLKLYSDRHYDLNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHMESDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIRVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRVVLSLHHPPDRTSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
92
Chicken
92
Rabbit
92
Zebrafish
90
Xenopus
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52948 As Substrate

Site PTM Type Enzyme
T168 Phosphorylation
T170 Phosphorylation
T172 Phosphorylation
K175 Ubiquitination
S179 Phosphorylation
T184 O-Glycosylation
K185 Ubiquitination
T190 Phosphorylation
K193 Ubiquitination
K198 Ubiquitination
R204 Methylation
K213 Ubiquitination
S511 Phosphorylation
S516 Phosphorylation
S524 Phosphorylation
K534 Ubiquitination
T536 Phosphorylation
K542 Ubiquitination
T545 Phosphorylation
T546 Phosphorylation
T548 Phosphorylation
Y550 Phosphorylation
K551 Ubiquitination
T553 Phosphorylation
K563 Ubiquitination
T567 O-Glycosylation
T568 O-Glycosylation
T568 Phosphorylation
K572 Acetylation
K572 Methylation
K572 Ubiquitination
K594 Acetylation
K594 Sumoylation
S596 Phosphorylation
K603 Acetylation
K603 Ubiquitination
S608 Phosphorylation Q9HC98 (NEK6)
S612 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S618 Phosphorylation
S623 Phosphorylation P06493 (CDK1)
S625 Phosphorylation
Y627 Phosphorylation
S635 Phosphorylation
S638 Phosphorylation
K639 Ubiquitination
S653 Phosphorylation
Y659 Phosphorylation
K665 Ubiquitination
T670 Phosphorylation P06493 (CDK1)
S673 Phosphorylation
K677 Ubiquitination
S679 Phosphorylation
S681 Phosphorylation
S683 Phosphorylation
T687 Phosphorylation
S704 Phosphorylation
S714 Phosphorylation
R719 Methylation
S726 Phosphorylation
Y741 Phosphorylation
Y742 Phosphorylation
T743 Phosphorylation
S746 Phosphorylation
K752 Ubiquitination
K757 Ubiquitination
K808 Ubiquitination
K818 Sumoylation
K818 Ubiquitination
K831 Ubiquitination
K838 Ubiquitination
S839 Phosphorylation Q9HC98 (NEK6)
R842 Methylation
Y848 Phosphorylation
S856 Phosphorylation
K858 Ubiquitination
K864 Ubiquitination
S888 Phosphorylation
S897 Phosphorylation
T899 Phosphorylation
S900 Phosphorylation
S913 Phosphorylation
T916 Phosphorylation
K925 Ubiquitination
S932 Phosphorylation
S934 Phosphorylation
S948 Phosphorylation
S966 Phosphorylation
S997 Phosphorylation
T1000 Phosphorylation
S1014 Phosphorylation
S1018 Phosphorylation
K1019 Sumoylation
K1019 Ubiquitination
T1022 Phosphorylation
S1023 Phosphorylation
S1028 Phosphorylation
S1034 Phosphorylation
S1036 Phosphorylation
S1038 Phosphorylation
K1040 Ubiquitination
T1041 Phosphorylation
S1043 Phosphorylation
K1052 Ubiquitination
T1054 Phosphorylation
S1055 Phosphorylation
S1060 Phosphorylation
S1062 Phosphorylation
S1064 Phosphorylation
T1070 Phosphorylation
S1075 Phosphorylation
S1099 Phosphorylation
K1128 Ubiquitination
S1174 Phosphorylation
S1192 Phosphorylation
K1200 Ubiquitination
S1202 Phosphorylation
K1207 Ubiquitination
K1213 Ubiquitination
Y1215 Phosphorylation
K1222 Ubiquitination
K1224 Ubiquitination
Y1296 Phosphorylation
K1341 Acetylation
K1341 Ubiquitination
S1344 Phosphorylation
S1363 Phosphorylation
K1405 Ubiquitination
K1414 Ubiquitination
K1425 Ubiquitination
S1465 Phosphorylation
K1497 Ubiquitination
Y1499 Phosphorylation
R1502 Methylation
Y1504 Phosphorylation
R1513 Methylation
Y1522 Phosphorylation
K1601 Ubiquitination
K1608 Ubiquitination
K1614 Ubiquitination
K1620 Ubiquitination
K1630 Ubiquitination
K1639 Ubiquitination
K1648 Ubiquitination
Y1664 Phosphorylation
S1689 Phosphorylation
K1722 Ubiquitination
K1739 Ubiquitination
K1749 Ubiquitination
T1768 Phosphorylation
S1769 Phosphorylation
S1771 Phosphorylation
T1772 Phosphorylation
S1803 Phosphorylation
T1805 Phosphorylation

Research Backgrounds

Function:

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).

PTMs:

Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.

Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation.

Subcellular Location:

Nucleus membrane>Peripheral membrane protein>Nucleoplasmic side. Nucleus>Nuclear pore complex. Nucleus>Nucleoplasm.
Note: Localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket (PubMed:11839768). Dissociates from the dissasembled NPC structure early during prophase of mitosis (PubMed:12802065). Colocalized with NUP153 and TPR to the nuclear basket of NPC (PubMed:11839768). Colocalized with DHX9 in diffuse and discrete intranuclear foci (GLFG-body) (PubMed:11839768, PubMed:28221134). Remains localized to the nuclear membrane after poliovirus (PV) infection (PubMed:11106761).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of the nuclear pore complex (NPC). Interacts directly with NUP96. Part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and NUP96; this complex plays a role in RNA export and in tethering NUP98 and NUP153 to the nucleus. Interacts with RAE1. Does not interact with TPR. Interacts with NUP88. Interacts directly with NUP88 and NUP214, subunits of the cytoplasmic filaments of the NPC (By similarity). Interacts (via N-terminus) with DHX9 (via DRBM, OB-fold and RGG domains); this interaction occurs in a RNA-dependent manner and stimulates DHX9-mediated ATPase activity.

(Microbial infection) Interacts with vesicular stomatitis virus protein M.

Family&Domains:

Contains G-L-F-G repeats. The FG repeat domains in Nup98 have a direct role in the transport.

Belongs to the nucleoporin GLFG family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > Influenza A.

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