METAP2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 3T3-L1 cells(heat-shock treatment), using METAP2 Antibody.

Product:	METAP2 Antibody
Catalog:	DF8406
Description:	Rabbit polyclonal antibody to METAP2
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	52 kDa; 53kD(Calculated).
Uniprot:	P50579
RRID:	AB_2841657

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)

Clonality:

Polyclonal

Specificity:

METAP2 Antibody detects endogenous levels of total METAP2.

RRID:

AB_2841657
Cite Format: Affinity Biosciences Cat# DF8406, RRID:AB_2841657.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A930035J23Rik; AI047573; AL024412; Amp2; AU014659,; EIF 2 associated p67 homolog; EIF-2-associated p67; Initiation factor 2 associated 67 kDa glycoprotein; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MAP2_HUMAN; MetAP 2; Metap2; Methionine aminopeptidase 2; Methionyl aminopeptidase 2; MGC102452; MGC127390; MGC53792; MNPEP; p67; p67eIF2; Peptidase M; Peptidase M2;

Immunogens

Immunogen:

Uniprot:

P50579(MAP2_HUMAN) >>Visit HPA database.

Gene(ID):

METAP2(10988)

Sequence:

P50579 MAP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50579 As Substrate

P50579

Site	PTM Type	Source
A2	Acetylation	Uniprot
R23	Methylation	Uniprot
S29	Phosphorylation	Uniprot
T30	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
S49	Phosphorylation	Uniprot
K58	Ubiquitination	Uniprot
S60	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
S74	Phosphorylation	Uniprot
K79	Ubiquitination	Uniprot
T96	Phosphorylation	Uniprot
K98	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
Y137	Phosphorylation	Uniprot
T145	Phosphorylation	Uniprot
K156	Ubiquitination	Uniprot
Y182	Phosphorylation	Uniprot
K278	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
K289	Ubiquitination	Uniprot
K323	Ubiquitination	Uniprot
K342	Ubiquitination	Uniprot
K348	Ubiquitination	Uniprot
T365	Phosphorylation	Uniprot
T369	Phosphorylation	Uniprot
K385	Ubiquitination	Uniprot
K427	Acetylation	Uniprot
K427	Ubiquitination	Uniprot
C436	S-Nitrosylation	Uniprot
C448	S-Nitrosylation	Uniprot

Research Backgrounds

P50579_MAP2_HUMAN

Function:

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.

Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

PTMs:

Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.

Subcellular Location:

Cytoplasm.
Note: About 30% of expressed METAP2 associates with polysomes.

Subunit Structure:

Interacts strongly with the eIF-2 gamma-subunit EIF2S3 (By similarity). Binds EIF2S1 at low magnesium concentrations.

Family&Domains:

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.

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METAP2 Antibody - #DF8406