ABCG5 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of ABCG5 using NIH-3T3 whole cell lysates.

Figure 1The effect of clinical ketosis on the abundance of key molecules in cholesterol synthesis, transport, and excretion.

Product:	ABCG5 Antibody
Catalog:	DF8401
Description:	Rabbit polyclonal antibody to ABCG5
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	72 kDa; 73kD(Calculated).
Uniprot:	Q9H222
RRID:	AB_2841653

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)

Clonality:

Polyclonal

Specificity:

ABCG5 Antibody detects endogenous levels of total ABCG5.

RRID:

AB_2841653
Cite Format: Affinity Biosciences Cat# DF8401, RRID:AB_2841653.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Abcg5; ABCG5_HUMAN; ATP binding cassette sub family G (WHITE) member 5 (sterolin 1); ATP binding cassette sub family G member 5; ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; Sterolin 1; Sterolin-1; STSL;

Immunogens

Immunogen:

Uniprot:

Q9H222(ABCG5_HUMAN) >>Visit HPA database.

Gene(ID):

ABCG5(64240)

Expression:

Q9H222 ABCG5_HUMAN:

Strongly expressed in the liver, lower levels in the small intestine and colon.

Sequence:

Q9H222 ABCG5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLGRAGTFLGEVYVNGRALRREQFQDCFSYVLQSDTLLSSLTVRETLHYTALLAIRRGNPGSFQKKVEAVMAELSLSHVADRLIGNYSLGGISTGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLVELARRNRIVVLTIHQPRSELFQLFDKIAILSFGELIFCGTPAEMLDFFNDCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETSKRVQMIESAYKKSAICHKTLKNIERMKHLKTLPMVPFKTKDSPGVFSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRSNVLKGAIQDRVGLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSSNVSVTTNPMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLISR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Zebrafish

Pig

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H222 As Substrate

Q9H222

Site	PTM Type	Source
S12	Phosphorylation	Uniprot
T108	Phosphorylation	Uniprot
Y114	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
T338	Phosphorylation	Uniprot
T350	Phosphorylation	Uniprot

Research Backgrounds

Q9H222_ABCG5_HUMAN

Function:

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Strongly expressed in the liver, lower levels in the small intestine and colon.

Subunit Structure:

Heterodimer with ABCG8.

Family&Domains:

The Walker motif (consensus sequence G-X-X-G-X-G-K-[ST]-T) is expected to bind ATP. Within this motif, the conserved Lys is essential for transport activity mediated by the heterodimer with ABCG8.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). β-sitosterol Alleviates High Fatty Acid-Induced Lipid Accumulation in Calf Hepatocytes by Regulating Cholesterol Metabolism. The Journal of steroid biochemistry and molecular biology, 2024 (PubMed: 38740074) [IF=4.1]

2). Role of sortilin 1 (SORT1) on fatty acid–mediated cholesterol metabolism in primary calf hepatocytes. Journal of Dairy Science, 2022 (PubMed: 35940922) [IF=3.5]

Application: WB Species: cows Sample: Liver tissue

Figure 1The effect of clinical ketosis on the abundance of key molecules in cholesterol synthesis, transport, and excretion. Liver tissue from healthy cows and cows with clinical ketosis was collected and digested for analysis of mRNA and protein abundance. (A) Relative mRNA abundance of SREBF2, HMGCR, ACAT2, ABCA1, ABCG5, and ABCG8; (B) representative western blots; and (C) relative protein abundance of SREBF2, HMGCR, ACAT2, ABCA1, and ABCG5. Comparisons among groups were calculated using a 2-tailed unpaired Student's t-test.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ABCG5 Antibody - #DF8401