PNLIP Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of PNLIP using Jurkat whole cell lysates.

DF8397 at 1/100 staining Human pancreatic cancer and para-carcinoma tissue by IHC-P.

Product:	PNLIP Antibody
Catalog:	DF8397
Description:	Rabbit polyclonal antibody to PNLIP
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	51 kDa; 51kD(Calculated).
Uniprot:	P16233
RRID:	AB_2841652

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(83%), Horse(83%), Sheep(92%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

PNLIP Antibody detects endogenous levels of total PNLIP.

RRID:

AB_2841652
Cite Format: Affinity Biosciences Cat# DF8397, RRID:AB_2841652.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

lipase, pancreatic; LIPP_HUMAN; Pancreatic lipase; Pancreatic triacylglycerol lipase; PL; PNLIP; PNLIPD; PTL; Triacylglycerol acylhydrolase;

Immunogens

Immunogen:

Uniprot:

P16233(LIPP_HUMAN) >>Visit HPA database.

Gene(ID):

PNLIP(5406)

Sequence:

P16233 LIPP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVAADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRIVGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDPSDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDASNFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWYNNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

Dog

Rabbit

Pig

Horse

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16233 As Substrate

P16233

Site	PTM Type	Source
Y58	Phosphorylation	Uniprot
T59	Phosphorylation	Uniprot
T185	Phosphorylation	Uniprot
K334	Acetylation	Uniprot
T335	Phosphorylation	Uniprot
Y420	Phosphorylation	Uniprot

Research Backgrounds

P16233_LIPP_HUMAN

Subcellular Location:

Secreted.

Family&Domains:

Belongs to the AB hydrolase superfamily. Lipase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PNLIP Antibody - #DF8397