EAAT3 Antibody - #DF8383
| Product: | EAAT3 Antibody |
| Catalog: | DF8383 |
| Description: | Rabbit polyclonal antibody to EAAT3 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 63 kDa; 57kD(Calculated). |
| Uniprot: | P43005 |
| RRID: | AB_2841644 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8383, RRID:AB_2841644.
Fold/Unfold
EAA3_HUMAN; EAAC 1; EAAC1; EAAT 3; Excitatory amino acid carrier 1; Excitatory amino acid carrier1; Excitatory amino acid transporter 3; Excitatory amino acid transporter3; Excitatory amino-acid carrier 1; GLUTAMATE TRANSPORTER, HIGH-AFFINITY; MEAAC 1; MEAAC1; Neuronal and epithelial glutamate transporter; REAAC 1; REAAC1; Slc1 a1; Slc1a 1; SLC1A1; Sodium dependent glutamate/aspartate transporter 3; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1; SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1; Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 member 1; Solute carrier family 1, member 1;
Immunogens
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
- P43005 EAA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPLIISSMITGVAALDSNVSGKIGLRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMNMTEESFTAVMTTAISKNKTKEYKIVGMYSDGINVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFRKLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43005 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y51 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot |
Research Backgrounds
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity).
Glycosylated.
Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Synaptosome. Early endosome membrane. Late endosome membrane. Recycling endosome membrane.
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
Homotrimer (Probable). Interacts with ARL6IP5. Interacts with SORCS2; this interaction is important for normal expression at the cell membrane (By similarity).
Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A1 subfamily.
Research Fields
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Digestive system > Protein digestion and absorption.
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