RanBP1 Antibody - #DF8377

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Product: RanBP1 Antibody
Catalog: DF8377
Description: Rabbit polyclonal antibody to RanBP1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P43487
RRID: AB_2841639

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RanBP1 Antibody detects endogenous levels of total RanBP1.
RRID:
AB_2841639
Cite Format: Affinity Biosciences Cat# DF8377, RRID:AB_2841639.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HTF9A; RAN binding protein 1; Ran specific GTPase activating protein; Ran-binding protein 1; Ran-specific GTPase-activating protein; RANBP1; RANG_HUMAN;

Immunogens

Immunogen:
Uniprot:

P43487(RANG_HUMAN) >>Visit HPA database.

Gene(ID):
RANBP1(5902)
Sequence:
MAAAKDTHEDHDTSTENTDESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKERGTGDVKLLKHKEKGAIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAIRFLNAENAQKFKTKFEECRKEIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
92
Sheep
92
Rabbit
92
Chicken
67
Xenopus
57
Zebrafish
40
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43487 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K5 Acetylation
T7 Phosphorylation
T13 Phosphorylation
S14 Phosphorylation
T15 Phosphorylation
T18 Phosphorylation
S21 Phosphorylation
S32 Phosphorylation
K39 Ubiquitination
K50 Ubiquitination
K54 Acetylation
S60 Phosphorylation
K68 Acetylation
K68 Methylation
K68 Ubiquitination
K76 Ubiquitination
K79 Acetylation
Y103 Phosphorylation
T105 Phosphorylation
K111 Acetylation
K111 Ubiquitination
K134 Acetylation
K134 Ubiquitination
K150 Acetylation
K150 Ubiquitination
K152 Acetylation
K154 Acetylation
K154 Ubiquitination
K160 Acetylation
K183 Acetylation
K183 Ubiquitination
S188 Phosphorylation
K190 Acetylation
K190 Sumoylation
K190 Ubiquitination
T193 Phosphorylation
K194 Acetylation
K200 Acetylation

Research Backgrounds

Function:

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN. Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1. Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP.

Subunit Structure:

Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1 (By similarity). Identified in a complex with nucleotide-free RAN and RCC1.

Family&Domains:

Belongs to the RANBP1 family.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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