SENP2 Antibody - #DF8373

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Product: SENP2 Antibody
Catalog: DF8373
Description: Rabbit polyclonal antibody to SENP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 68 kDa; 68kD(Calculated).
Uniprot: Q9HC62
RRID: AB_2841635

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:200-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
SENP2 Antibody detects endogenous levels of total SENP2.
RRID:
AB_2841635
Cite Format: Affinity Biosciences Cat# DF8373, RRID:AB_2841635.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Axam2; DKFZp762A2316; KIAA1331; Senp2; SENP2_HUMAN; Sentrin specific protease 2; Sentrin-specific protease 2; Sentrin/SUMO specific protease SENP2; Sentrin/SUMO-specific protease SENP2; SMT3 specific isopeptidase 2; SMT3-specific isopeptidase 2; Smt3ip2; SUMO1/sentrin/SMT3 specific peptidase 2;

Immunogens

Immunogen:
Uniprot:

Q9HC62(SENP2_HUMAN) >>Visit HPA database.

Gene(ID):
SENP2(59343)
Sequence:
MYRWLVRILGTIFRFCDRSVPPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNAASLFGFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLGNKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKYRKLLERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRLVETRGPLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQLEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRRTDDLLELTEDMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYMNLLVERNKKQGYPALHVFSTFFYPKLKSGGYQAVKRWTKGVNLFEQEIILVPIHRKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDESKTKRNSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYISRDKPITFTQHQMPLFRKKMVWEILHQQLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Chicken
50
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC62 As Substrate

Site PTM Type Enzyme
S32 Phosphorylation
S34 Phosphorylation
T35 Phosphorylation
S123 Phosphorylation
Y130 Phosphorylation
S168 Phosphorylation
Y216 Phosphorylation
K224 Ubiquitination
S231 Phosphorylation
Y239 Phosphorylation
K253 Acetylation
K264 Acetylation
K271 Acetylation
K271 Ubiquitination
S284 Phosphorylation
S287 Phosphorylation
S292 Phosphorylation
S333 Phosphorylation
S344 Phosphorylation O14757 (CHEK1)
T369 Phosphorylation Q15418 (RPS6KA1)
K455 Ubiquitination
Y493 Phosphorylation
S496 Phosphorylation
Y558 Phosphorylation

Research Backgrounds

Function:

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB.

PTMs:

Polyubiquitinated; which leads to proteasomal degradation.

Subcellular Location:

Nucleus>Nuclear pore complex. Nucleus membrane>Peripheral membrane protein>Nucleoplasmic side. Cytoplasm.
Note: Shuttles between cytoplasm and nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds to SUMO2 and SUMO3 (By similarity). Interacts with the C-terminal domain of NUP153 via its N-terminus. Interacts with MTA1.

Family&Domains:

The N-terminus is necessary and sufficient for nuclear envelope targeting.

Belongs to the peptidase C48 family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

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