WRN Antibody - #DF8369

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Product: WRN Antibody
Catalog: DF8369
Description: Rabbit polyclonal antibody to WRN
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 162 kDa; 162kD(Calculated).
Uniprot: Q14191
RRID: AB_2841633

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
WRN Antibody detects endogenous levels of total WRN.
RRID:
AB_2841633
Cite Format: Affinity Biosciences Cat# DF8369, RRID:AB_2841633.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686C2056; DNA helicase; DNA helicase, RecQ like type 3; Exonuclease WRN; HGNC 12791; OTTHUMP00000225301; RecQ protein-like 2; RecQ-like type 3; RecQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome helicase; Werner syndrome protein; Werner syndrome, RecQ helicase like; WRN; WRN_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q14191(WRN_HUMAN) >>Visit HPA database.

Gene(ID):
WRN(7486)
Sequence:
MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVRKSVFEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFEDSTTGGVQQKQIREHEVLIHVEDETWDPTLDHLAKHDGEDVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQSQEEYLSDIAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNDNENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLPTKEEEEDDENEANEGEEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRDSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLVEVSRYNKFMKICALTKKGRNWLHKANTESQSLILQANEELCPKKLLLPSSKTVSSGTKEHCYNQVPVELSTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSQPVISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPGSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS

PTMs - Q14191 As Substrate

Site PTM Type Enzyme
S2 Acetylation
K5 Ubiquitination
K14 Ubiquitination
K32 Acetylation
K131 Ubiquitination
K135 Ubiquitination
K146 Ubiquitination
K154 Ubiquitination
K167 Ubiquitination
K187 Ubiquitination
K202 Ubiquitination
K241 Sumoylation
K241 Ubiquitination
S248 Phosphorylation
S282 Phosphorylation
K286 Ubiquitination
S319 Phosphorylation P78527 (PRKDC)
K356 Sumoylation
K366 Acetylation
K366 Ubiquitination
K382 Ubiquitination
S409 Phosphorylation
S426 Phosphorylation
S435 Phosphorylation
S440 Phosphorylation P78527 (PRKDC)
S453 Phosphorylation
S462 Phosphorylation
S467 Phosphorylation P78527 (PRKDC)
S478 Phosphorylation
K493 Acetylation
K493 Ubiquitination
K496 Sumoylation
K506 Ubiquitination
S689 Phosphorylation
T692 Phosphorylation
K775 Ubiquitination
T777 Phosphorylation
S790 Phosphorylation
S801 Phosphorylation
Y849 Phosphorylation
K887 Acetylation
K898 Sumoylation
K924 Ubiquitination
K934 Ubiquitination
S948 Phosphorylation
S968 Phosphorylation
K1036 Sumoylation
S1058 Phosphorylation
K1073 Ubiquitination
S1079 Phosphorylation
K1080 Ubiquitination
S1084 Phosphorylation
K1087 Ubiquitination
T1100 Phosphorylation
K1102 Ubiquitination
Y1110 Phosphorylation
S1111 Phosphorylation
Y1112 Phosphorylation
K1117 Acetylation
S1120 Phosphorylation
K1127 Acetylation
S1128 Phosphorylation
S1133 Phosphorylation
S1141 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
K1170 Ubiquitination
K1189 Ubiquitination
K1199 Ubiquitination
S1205 Phosphorylation
K1270 Ubiquitination
K1274 Acetylation
S1292 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
K1314 Ubiquitination
K1331 Ubiquitination
K1371 Ubiquitination
S1378 Phosphorylation
K1386 Ubiquitination
S1388 Phosphorylation
K1389 Acetylation
K1389 Ubiquitination
S1400 Phosphorylation
K1413 Acetylation
R1427 Methylation

Research Backgrounds

Function:

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.

PTMs:

Phosphorylated by PRKDC.

Subcellular Location:

Nucleus>Nucleolus. Nucleus. Nucleus>Nucleoplasm.
Note: Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1 (By similarity). Interacts with EXO1, PCNA and SUPV3L1. Interacts with PML (isoform PML-4).

Family&Domains:

Belongs to the helicase family. RecQ subfamily.

References

1). YTHDF1-CLOCK Axis Contributes to Pathogenesis of Allergic Airway Inflammation Through LLPS. , 2023

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