Integrin beta 5 Antibody - #AF0185

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Product: Integrin beta 5 Antibody
Catalog: AF0185
Description: Rabbit polyclonal antibody to Integrin beta 5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 100,130kD; 88kD(Calculated).
Uniprot: P18084
RRID: AB_2833378

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Integrin beta 5 Antibody detects endogenous levels of total Integrin beta 5.
RRID:
AB_2833378
Cite Format: Affinity Biosciences Cat# AF0185, RRID:AB_2833378.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ26658; Integrin beta-5; Integrin subunit beta 5; integrin, beta 5; ITB5_HUMAN; Itgb5; Testis secretory sperm-binding protein Li 217p;

Immunogens

Immunogen:
Uniprot:

P18084(ITB5_HUMAN) >>Visit HPA database.

Gene(ID):
ITGB5(3693)
Description:
ITGB5 Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Belongs to the integrin beta chain family. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V.
Sequence:
MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDLYYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPNCVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDVPHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTALIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGDTASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEFGKIYGPFCECDNFSCARNKGVLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQICSERGHCLCGQCQCTEPGAFGEMCEKCPTCPDACSTKRDCVECLLLHSGKPDNQTCHSLCRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELPSGKSNLTVLREPECGNTPNAMTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMASNPLYRKPISTHTVDFTFNKFNKSYNGTVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18084 As Substrate

Site PTM Type Enzyme
T61 Phosphorylation
S188 Phosphorylation
T199 O-Glycosylation
K206 Acetylation
K260 Ubiquitination
T656 Phosphorylation
S659 Phosphorylation
K685 Ubiquitination
K756 Ubiquitination
S759 Phosphorylation O96013 (PAK4)
S762 Phosphorylation O96013 (PAK4)
Y766 Phosphorylation
S770 Phosphorylation
Y774 Phosphorylation
K776 Ubiquitination
S779 Phosphorylation
T780 Phosphorylation
T782 Phosphorylation
T786 Phosphorylation
K792 Ubiquitination
S793 Phosphorylation
T797 Phosphorylation

Research Backgrounds

Function:

Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV). Interacts with MYO10. Interacts with DAB2. Integrin ITGAV:ITGB5 interacts with FBLN5 (via N-terminus) (By similarity). ITGAV:ITGB5 interacts with CCN3.

(Microbial infection) Integrin ITGAV:ITGB5 interacts with adenovirus type C penton protein.

Family&Domains:

Belongs to the integrin beta chain family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

References

1). Convallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPARγ-Integrin αvβ5 Signaling Pathway. Drug Design Development and Therapy, 2021 (PubMed: 33654384) [IF=4.8]

Application: WB    Species: Mice    Sample: aortic tissues and RAW264.7 cells

Figure 6 PPARγ antagonist, GW9662, blocked CNT-enhanced M2 macrophage polarization. (A and B) Western blot analysis and quantification of PPARγ, Integrin αv and Integrin β5 in the aortic tissues (A) and RAW264.7 cells (B). (C) Representative images of Oil red O staining in macrophages. Bar=50 μm. (D) Levels of TC in cells were measured by available kits. (E) Arg1, Mrc1, Retnla and Chi3l3 mRNA levels were assessed by qPCR. (F) IL-6, TNF-α and IL-10 contents in cell supernatants were tested using ELISA. Data were expressed as mean ± SD.

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