UBA3 Antibody - #DF8358

(3)  
Product: UBA3 Antibody
Catalog: DF8358
Description: Rabbit polyclonal antibody to UBA3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 49 kDa; 52kD(Calculated).
Uniprot: Q8TBC4
RRID: AB_2841623

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
UBA3 Antibody detects endogenous levels of total UBA3.
RRID:
AB_2841623
Cite Format: Affinity Biosciences Cat# DF8358, RRID:AB_2841623.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp566J164; EC 6.3.2.; hUba3; MGC22384; NEDD8 activating enzyme E1 catalytic subunit; NEDD8 activating enzyme E1C; Nedd8 activating enzyme hUba3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; uba3; UBA3 ubiquitin activating enzyme E1 homolog; UBA3_HUMAN; UBE1C; Ubiquitin activating enzyme 3; Ubiquitin activating enzyme E1C; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3;

Immunogens

Immunogen:
Uniprot:

Q8TBC4(UBA3_HUMAN) >>Visit HPA database.

Gene(ID):
UBA3(9039)
Expression:
Q8TBC4 UBA3_HUMAN:

Ubiquitously expressed.

Sequence:
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
80
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TBC4 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K9 Methylation
K10 Methylation
K21 Ubiquitination
K86 Ubiquitination
S91 Phosphorylation
T103 Phosphorylation
S107 Phosphorylation
K124 Ubiquitination
R135 Methylation
K147 Sumoylation
K147 Ubiquitination
Y185 Phosphorylation
T203 Phosphorylation
K207 Sumoylation
K207 Ubiquitination
K260 Ubiquitination
K284 Sumoylation
K284 Ubiquitination
R295 Methylation
K307 Ubiquitination
K361 Ubiquitination
S377 Phosphorylation
T390 Phosphorylation
S392 Phosphorylation
S394 Phosphorylation
K398 Ubiquitination
S399 Phosphorylation
K409 Acetylation
K409 Ubiquitination
K434 Ubiquitination

Research Backgrounds

Function:

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity). Interacts with TBATA (By similarity).

Family&Domains:

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.