DDIT4 Antibody - #DF8353
| Product: | DDIT4 Antibody |
| Catalog: | DF8353 |
| Description: | Rabbit polyclonal antibody to DDIT4 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 25 kDa; 25kD(Calculated). |
| Uniprot: | Q9NX09 |
| RRID: | AB_2841618 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8353, RRID:AB_2841618.
Fold/Unfold
DDIT4; DDIT4_HUMAN; Dig2; DNA damage inducible transcript 4; DNA damage inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; FLJ20500; HIF 1 responsive protein RTP801; HIF 1 responsive RTP801; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; REDD1; RTP801;
Immunogens
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
- Q9NX09 DDIT4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NX09 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| T23 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot |
Research Backgrounds
Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
Phosphorylated by GSK3B; this promotes proteasomal degradation.
Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.
Mitochondrion. Cytoplasm>Cytosol.
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
Monomer. Interacts with BTRC. Identified in a complex with CUL4A, DDB1 and BTRC. Interacts with TXNIP; this inhibits the proteasomal degradation of DDIT4.
Belongs to the DDIT4 family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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