IL22 Antibody | Affinity Biosciences

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Figure 3 Chlamydia muridarum upregulates IL-22 and occludin expression in mice with dextran sodium sulfate- (DSS-) induced colitis.

Product:	IL22 Antibody
Catalog:	DF8343
Description:	Rabbit polyclonal antibody to IL22
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Dog
Mol.Wt.:	20 kDa; 20kD(Calculated).
Uniprot:	Q9GZX6
RRID:	AB_2841610

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

IL22 Antibody detects endogenous levels of total IL22.

RRID:

AB_2841610
Cite Format: Affinity Biosciences Cat# DF8343, RRID:AB_2841610.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cytokine Zcyto18; IL 10 related T cell derived inducible factor; IL 21; IL 22; IL D110; IL TIF; IL-10-related T-cell-derived-inducible factor; IL-22; IL-TIF; IL21; Il22; IL22_HUMAN; ILD110; ILTIF; Interleukin 10 related T cell derived inducible factor; interleukin 21; Interleukin 22; Interleukin-22; MGC79382; MGC79384; TIFa; TIFIL 23; TIFIL23; UNQ3099/PRO10096; zcyto18;

Immunogens

Immunogen:

Uniprot:

Q9GZX6(IL22_HUMAN) >>Visit HPA database.

Gene(ID):

IL22(50616)

Sequence:

Q9GZX6 IL22_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALQKSVSSFLMGTLATSCLLLLALLVQGGAAAPISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Rabbit

Chicken

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZX6 As Substrate

Q9GZX6

Site	PTM Type	Source
N54	N-Glycosylation	Uniprot
N97	N-Glycosylation	Uniprot
S126	Phosphorylation	Uniprot

Research Backgrounds

Q9GZX6_IL22_HUMAN

Function:

Cytokine that contributes to the inflammatory response in vivo.

Subcellular Location:

Secreted.

Family&Domains:

Belongs to the IL-10 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

References

1). Th22 cells induce Müller cell activation via the Act1/TRAF6 pathway in diabetic retinopathy. CELL AND TISSUE RESEARCH, 2022 (PubMed: 36201050) [IF=3.6]

2). Chlamydia muridarum Alleviates Colitis via the IL-22/Occludin Signal Pathway. Biomed Research International, 2020 (PubMed: 33381598)

Application: WB Species: Mice Sample: colon tissue

Figure 3 Chlamydia muridarum upregulates IL-22 and occludin expression in mice with dextran sodium sulfate- (DSS-) induced colitis. (a) IL-22 and occludin expression in various groups assessed using western blotting. (b) Relative protein expression of IL-22 and occludin. (n = 3 per group). ∗P < 0.05, DSS group compared with control group; #P < 0.05, CM+DSS group compared with DSS group; &P < 0.05, CM+DSS+aIL-22 group compared with CM+DSS group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IL22 Antibody - #DF8343