Product: IL22 Antibody
Catalog: DF8343
Description: Rabbit polyclonal antibody to IL22
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 20 kDa; 20kD(Calculated).
Uniprot: Q9GZX6
RRID: AB_2841610

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IL22 Antibody detects endogenous levels of total IL22.
RRID:
AB_2841610
Cite Format: Affinity Biosciences Cat# DF8343, RRID:AB_2841610.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cytokine Zcyto18; IL 10 related T cell derived inducible factor; IL 21; IL 22; IL D110; IL TIF; IL-10-related T-cell-derived-inducible factor; IL-22; IL-TIF; IL21; Il22; IL22_HUMAN; ILD110; ILTIF; Interleukin 10 related T cell derived inducible factor; interleukin 21; Interleukin 22; Interleukin-22; MGC79382; MGC79384; TIFa; TIFIL 23; TIFIL23; UNQ3099/PRO10096; zcyto18;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAALQKSVSSFLMGTLATSCLLLLALLVQGGAAAPISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
88
Horse
79
Rabbit
74
Chicken
67
Bovine
63
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZX6 As Substrate

Site PTM Type Enzyme
N54 N-Glycosylation
N97 N-Glycosylation
S126 Phosphorylation

Research Backgrounds

Function:

Cytokine that contributes to the inflammatory response in vivo.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the IL-10 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

References

1). Th22 cells induce Müller cell activation via the Act1/TRAF6 pathway in diabetic retinopathy. CELL AND TISSUE RESEARCH, 2022 (PubMed: 36201050) [IF=3.6]

2). Chlamydia muridarum Alleviates Colitis via the IL-22/Occludin Signal Pathway. Biomed Research International, 2020 (PubMed: 33381598)

Application: WB    Species: Mice    Sample: colon tissue

Figure 3 Chlamydia muridarum upregulates IL-22 and occludin expression in mice with dextran sodium sulfate- (DSS-) induced colitis. (a) IL-22 and occludin expression in various groups assessed using western blotting. (b) Relative protein expression of IL-22 and occludin. (n = 3 per group). ∗P < 0.05, DSS group compared with control group; #P < 0.05, CM+DSS group compared with DSS group; &P < 0.05, CM+DSS+aIL-22 group compared with CM+DSS group.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.