TXNRD1 Antibody - #DF8339

(3)  
Product: TXNRD1 Antibody
Catalog: DF8339
Description: Rabbit polyclonal antibody to TXNRD1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 55 kDa; 71kD(Calculated).
Uniprot: Q16881
RRID: AB_2841607

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TXNRD1 Antibody detects endogenous levels of total TXNRD1.
RRID:
AB_2841607
Cite Format: Affinity Biosciences Cat# DF8339, RRID:AB_2841607.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytoplasmic; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; Gene associated with retinoid IFN induced mortality 12 protein; GRIM 12; GRIM-12; GRIM12; KDRF; KM 102 derived reductase like factor; KM-102-derived reductase-like factor; MGC9145; Oxidoreductase; Thioredoxin reductase 1; Thioredoxin reductase 1 cytoplasmic; Thioredoxin reductase GRIM 12; Thioredoxin reductase TR1; TR 1; TR; TR1; TRXR 1; TRXR1; TRXR1_HUMAN; TXNR; TXNRD 1; Txnrd1;

Immunogens

Immunogen:

A synthesized peptide derived from human TXNRD1, corresponding to a region within the internal amino acids.

Uniprot:

Q16881(TRXR1_HUMAN) >>Visit HPA database.

Gene(ID):
TXNRD1(7296)
Expression:
Q16881 TRXR1_HUMAN:

Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

Sequence:
MGCAEGKAVAAAAPTELQTKGKNGDGRRRSAKDHHPGKTLPENPAGFTSTATADSRALLQAYIDGHSVVIFSRSTCTRCTEVKKLFKSLCVPYFVLELDQTEDGRALEGTLSELAAETDLPVVFVKQRKIGGHGPTLKAYQEGRLQKLLKMNGPEDLPKSYDYDLIIIGGGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCUG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16881 As Substrate

Site PTM Type Enzyme
T39 Phosphorylation
T50 Phosphorylation
K126 Ubiquitination
K129 Ubiquitination
K138 Methylation
K138 Ubiquitination
K147 Ubiquitination
K150 Methylation
S160 Phosphorylation
Y161 Phosphorylation
Y163 Phosphorylation
K179 Ubiquitination
Y184 Phosphorylation
K187 Ubiquitination
T195 Phosphorylation
T197 Phosphorylation
K217 Ubiquitination
K218 Ubiquitination
K239 Acetylation
K239 Ubiquitination
K245 Acetylation
K245 Ubiquitination
K274 Ubiquitination
Y277 Phosphorylation
Y281 Phosphorylation
K291 Ubiquitination
K298 Acetylation
K300 Ubiquitination
K324 Ubiquitination
K385 Ubiquitination
K396 Ubiquitination
K405 Acetylation
K405 Sumoylation
K405 Ubiquitination
T413 Phosphorylation
Y434 Phosphorylation
K449 Ubiquitination
K458 Ubiquitination
K462 Ubiquitination
K465 Ubiquitination
T469 Phosphorylation
T474 Phosphorylation
Y478 Phosphorylation
K489 Ubiquitination
T493 Phosphorylation
K514 Ubiquitination
K539 Ubiquitination
Y555 Phosphorylation
Y572 Phosphorylation
K574 Ubiquitination
K580 Ubiquitination
K607 Ubiquitination
K613 Ubiquitination
C647 S-Nitrosylation

Research Backgrounds

Function:

Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

PTMs:

The N-terminus of isoform 5 is blocked.

ISGylated.

Subcellular Location:

Cytoplasm.

Cytoplasm. Nucleus.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

Subunit Structure:

Homodimer. Isoform 4 interacts with ESR1 and ESR2. Interacts with HERC5.

Family&Domains:

The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

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