Product: BDKRB2 Antibody
Catalog: DF8337
Description: Rabbit polyclonal antibody to BDKRB2
Application: WB
Reactivity: Human
Prediction: Dog
Mol.Wt.: 78 kDa; 44kD(Calculated).
Uniprot: P30411
RRID: AB_2841605

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
BDKRB2 Antibody detects endogenous levels of total BDKRB2.
RRID:
AB_2841605
Cite Format: Affinity Biosciences Cat# DF8337, RRID:AB_2841605.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B2; B2 bradykinin receptor; B2BKR; B2BRA; B2R; BDKR B2; BDKRB 2; BDKRB2; BK 2; BK 2 receptor; BK R2; BK-2 receptor; BK2; BK2 receptor; BK2R; BKR 2; BKR2; BKRB2_HUMAN; BR B2; Bradykinin receptor B2; Bradykinin receptor beta 2; BRB 2; BRB2; DKFZp686O088; Kinin B2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P30411 BKRB2_HUMAN:

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

Sequence:
MFSPWKISMFLSVREDSVPTTASFSADMLNVTLQGPTLNGTFAQSKCPQVEWLGWLNTIQPPFLWVLFVLATLENIFVLSVFCLHKSSCTVAEIYLGNLAAADLILACGLPFWAITISNNFDWLFGETLCRVVNAIISMNLYSSICFLMLVSIDRYLALVKTMSMGRMRGVRWAKLYSLVIWGCTLLLSSPMLVFRTMKEYSDEGHNVTACVISYPSLIWEVFTNMLLNVVGFLLPLSVITFCTMQIMQVLRNNEMQKFKEIQTERRATVLVLVVLLLFIICWLPFQISTFLDTLHRLGILSSCQDERIIDVITQIASFMAYSNSCLNPLVYVIVGKRFRKKSWEVYQGVCQKGGCRSEPIQMENSMGTLRTSISVERQIHKLQDWAGSRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Pig
75
Horse
75
Bovine
75
Sheep
75
Rabbit
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30411 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
T162 Phosphorylation
Y177 Phosphorylation P12931 (SRC)
S178 Phosphorylation
Y332 Phosphorylation
Y347 Phosphorylation P12931 (SRC)
S358 Phosphorylation
S366 Phosphorylation P32298 (GRK4) , P35626 (GRK3) , P25098 (GRK2) , P34947 (GRK5) , P43250 (GRK6)
S373 Phosphorylation P43250 (GRK6) , P25098 (GRK2) , P35626 (GRK3) , P17252 (PRKCA) , Q05655 (PRKCD) , P32298 (GRK4) , P34947 (GRK5)
S375 Phosphorylation P34947 (GRK5) , P35626 (GRK3) , P32298 (GRK4) , P25098 (GRK2) , P43250 (GRK6)
K382 Ubiquitination

Research Backgrounds

Function:

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

Subunit Structure:

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

References

1). Roles for heterodimerization of APJ and B2R in promoting cell proliferation via ERK1/2-eNOS signaling pathway. CELLULAR SIGNALLING, 2020 (PubMed: 32407761) [IF=4.8]

Application: WB    Species: human    Sample: HUVECs and HEK293 cells

Fig. 1. | Co-expression and co-localization of APJ and B2R in HUVECs and HEK293 cells. (A) APJ (left, 43KD) and B2R (right, 45KD) expression in HUVECs and HEK293 cells were detected by western blotting.

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For Research Use Only.
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