BDKRB2 Antibody | Affinity Biosciences

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Product:	BDKRB2 Antibody
Catalog:	DF8337
Description:	Rabbit polyclonal antibody to BDKRB2
Application:	WB
Reactivity:	Human
Prediction:	Dog
Mol.Wt.:	78 kDa; 44kD(Calculated).
Uniprot:	P30411
RRID:	AB_2841605

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Dog(83%)

Clonality:

Polyclonal

Specificity:

BDKRB2 Antibody detects endogenous levels of total BDKRB2.

RRID:

AB_2841605
Cite Format: Affinity Biosciences Cat# DF8337, RRID:AB_2841605.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B2; B2 bradykinin receptor; B2BKR; B2BRA; B2R; BDKR B2; BDKRB 2; BDKRB2; BK 2; BK 2 receptor; BK R2; BK-2 receptor; BK2; BK2 receptor; BK2R; BKR 2; BKR2; BKRB2_HUMAN; BR B2; Bradykinin receptor B2; Bradykinin receptor beta 2; BRB 2; BRB2; DKFZp686O088; Kinin B2;

Immunogens

Immunogen:

Uniprot:

P30411(BKRB2_HUMAN) >>Visit HPA database.

Gene(ID):

BDKRB2(624)

Expression:

P30411 BKRB2_HUMAN:

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

Sequence:

P30411 BKRB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFSPWKISMFLSVREDSVPTTASFSADMLNVTLQGPTLNGTFAQSKCPQVEWLGWLNTIQPPFLWVLFVLATLENIFVLSVFCLHKSSCTVAEIYLGNLAAADLILACGLPFWAITISNNFDWLFGETLCRVVNAIISMNLYSSICFLMLVSIDRYLALVKTMSMGRMRGVRWAKLYSLVIWGCTLLLSSPMLVFRTMKEYSDEGHNVTACVISYPSLIWEVFTNMLLNVVGFLLPLSVITFCTMQIMQVLRNNEMQKFKEIQTERRATVLVLVVLLLFIICWLPFQISTFLDTLHRLGILSSCQDERIIDVITQIASFMAYSNSCLNPLVYVIVGKRFRKKSWEVYQGVCQKGGCRSEPIQMENSMGTLRTSISVERQIHKLQDWAGSRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30411 As Substrate

P30411

Site	PTM Type	Enzyme	Source
S17	Phosphorylation		Uniprot
T162	Phosphorylation		Uniprot
Y177	Phosphorylation	P12931 (SRC)	Uniprot
S178	Phosphorylation		Uniprot
Y332	Phosphorylation		Uniprot
Y347	Phosphorylation	P12931 (SRC)	Uniprot
S358	Phosphorylation		Uniprot
S366	Phosphorylation	P32298 (GRK4) , P35626 (GRK3) , P25098 (GRK2) , P34947 (GRK5) , P43250 (GRK6)	Uniprot
S373	Phosphorylation	P43250 (GRK6) , P25098 (GRK2) , P35626 (GRK3) , P17252 (PRKCA) , Q05655 (PRKCD) , P32298 (GRK4) , P34947 (GRK5)	Uniprot
S375	Phosphorylation	P34947 (GRK5) , P35626 (GRK3) , P32298 (GRK4) , P25098 (GRK2) , P43250 (GRK6)	Uniprot
K382	Ubiquitination		Uniprot

Research Backgrounds

P30411_BKRB2_HUMAN

Function:

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

Subunit Structure:

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

References

1). Roles for heterodimerization of APJ and B2R in promoting cell proliferation via ERK1/2-eNOS signaling pathway. CELLULAR SIGNALLING, 2020 (PubMed: 32407761) [IF=4.8]

Application: WB Species: human Sample: HUVECs and HEK293 cells

Fig. 1. | Co-expression and co-localization of APJ and B2R in HUVECs and HEK293 cells. (A) APJ (left, 43KD) and B2R (right, 45KD) expression in HUVECs and HEK293 cells were detected by western blotting.

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BDKRB2 Antibody - #DF8337