Product: KRT12 Antibody
Catalog: DF8321
Description: Rabbit polyclonal antibody to KRT12
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: Q99456
RRID: AB_2841597

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(82%), Sheep(82%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
KRT12 Antibody detects endogenous levels of total KRT12.
RRID:
AB_2841597
Cite Format: Affinity Biosciences Cat# DF8321, RRID:AB_2841597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK-12; Cytokeratin-12; K12; K1C12_HUMAN; Keratin 12; Keratin; Keratin, type I cytoskeletal 12; Keratin-12; KRT12; type I cytoskeletal 12;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99456 K1C12_HUMAN:

Cornea specific.

Sequence:
MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSASSVGSGYGGSAFGFGASCGGGFSAASMFGSSSGFGGGSGSSMAGGLGAGYGRALGGGSFGGLGMGFGGSPGGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYLDKVRALEEANTELENKIREWYETRGTGTADASQSDYSKYYPLIEDLRNKIISASIGNAQLLLQIDNARLAAEDFRMKYENELALRQGVEADINGLRRVLDELTLTRTDLEMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Pig
82
Bovine
82
Sheep
82
Dog
82
Horse
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99456 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
S138 Phosphorylation
Y139 Phosphorylation
K142 Acetylation
K142 Ubiquitination
Y161 Phosphorylation
T163 Phosphorylation
K189 Ubiquitination
Y262 Phosphorylation
S343 Phosphorylation
Y429 Phosphorylation

Research Backgrounds

Function:

May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).

Tissue Specificity:

Cornea specific.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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For Research Use Only.
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