PANX1 Antibody | Affinity Biosciences

IF/ICC
Cites

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DF8316 staining Hela cells by IF/ICC.

Figure 2.

Figure 3.

Product:	PANX1 Antibody
Catalog:	DF8316
Description:	Rabbit polyclonal antibody to PANX1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q96RD7
RRID:	AB_2841594

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

PANX1 Antibody detects endogenous levels of total PANX1.

RRID:

AB_2841594
Cite Format: Affinity Biosciences Cat# DF8316, RRID:AB_2841594.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

innexin; MRS1; Pannexin 1; Pannexin-1; Panx1; PANX1_HUMAN; PX1; UNQ2529;

Immunogens

Immunogen:

Uniprot:

Q96RD7(PANX1_HUMAN) >>Visit HPA database.

Gene(ID):

PANX1(24145)

Sequence:

Q96RD7 PANX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC

PTMs - Q96RD7 As Substrate

Q96RD7

Site	PTM Type	Source
Y10	Phosphorylation	Uniprot
Y150	Phosphorylation	Uniprot
S182	Phosphorylation	Uniprot
S189	Phosphorylation	Uniprot
Y199	Phosphorylation	Uniprot
K201	Ubiquitination	Uniprot
K203	Acetylation	Uniprot
K204	Acetylation	Uniprot
K212	Acetylation	Uniprot
N255	N-Glycosylation	Uniprot
K307	Ubiquitination	Uniprot
K343	Ubiquitination	Uniprot
K355	Ubiquitination	Uniprot
K381	Ubiquitination	Uniprot
S385	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
S425	Phosphorylation	Uniprot

Research Backgrounds

Q96RD7_PANX1_HUMAN

Function:

Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.

PTMs:

S-nitrosylation inhibits channel currents and ATP release.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Subunit Structure:

Homohexamer. Forms homomeric or PANX1/PANX2-heteromeric intercellular channels on coexpression in paired Xenopus oocytes (By similarity).

Family&Domains:

Belongs to the pannexin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

References

1). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: WB Species: Rat Sample:

Figure 2. Px1 and Cx43 expression in the spinal cords of IP model rats. Expression of Px1 and Cx42 in rats was detected via western blotting at (A) 2, (B) 4, (C) 6 and (D) 24 h post-surgery. The scanning density of (E) Px1 and (F) Cx43 expression normalized to control. *P<0.05, **P<0.01 and ***P<0.001 vs. C group; #P<0.05, ##P<0.01 and ###P<0.001 vs. NS group; ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. CBX group. Data are presented as the mean ± SD. C, control group; IP, incision pain; NS, normal saline; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; Cx43, connexin 43.

Application: IF/ICC Species: Rat Sample:

Figure 3. Co-localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, ×200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; GFAP, glial fibrillary acidic protein.

Application: IF/ICC Species: rat Sample:

Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red)expression was analyzed using an immunofluorescence assay.Magnification, x200; scale bar, 10 µm. C,control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein

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PANX1 Antibody - #DF8316